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2017-09-26Zeitschriftenartikel DOI: 10.3389/fmicb.2017.01888
Use of RNALater® Preservation for Virome Sequencing in Outbreak Settings
dc.contributor.authorKohl, Claudia
dc.contributor.authorWegener, Merle
dc.contributor.authorNitsche, Andreas
dc.contributor.authorKurth, Andreas
dc.date.accessioned2018-05-07T20:39:04Z
dc.date.available2018-05-07T20:39:04Z
dc.date.created2017-10-05
dc.date.issued2017-09-26none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reE7yue6stg/PDF/25d8ngLlJFu0U.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2843
dc.description.abstractOutbreaks of infectious diseases may occur in animal and human populations; this underlines the need for suitable One Health approaches. During outbreak situations, straightforward identification of etiological agents is indispensable for taking countermeasures. A recently published protocol for metagenomic virus detection in clinical specimens (TUViD-VM) was developed for snap-frozen tissues which can be challenging to obtain. Here, we describe the use of RNALater®-treated tissue at ambient temperatures for virome sequencing. This study demonstrates that samples stored in RNALater® buffer yield similar results to those stored snap-frozen.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subject.ddc610 Medizin
dc.titleUse of RNALater® Preservation for Virome Sequencing in Outbreak Settings
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10055289
dc.identifier.doi10.3389/fmicb.2017.01888
dc.identifier.doihttp://dx.doi.org/10.25646/2768
local.edoc.container-titleFrontiers in Microbiology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2017.01888/full
local.edoc.container-publisher-nameFrontiers Media
local.edoc.container-volume8
local.edoc.container-issue1888
local.edoc.container-year2017

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