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2017-12-22Zeitschriftenartikel DOI: 10.1186/s12917-017-1307-x
Coronavirus and paramyxovirus in bats from Northwest Italy
dc.contributor.authorRizzo, Francesca
dc.contributor.authorEdenborough, Kathryn M.
dc.contributor.authorToffoli, Roberto
dc.contributor.authorCulasso, Paola
dc.contributor.authorZoppi, Simona
dc.contributor.authorDondo, Alessandro
dc.contributor.authorRobetto, Serena
dc.contributor.authorRosati, Sergio
dc.contributor.authorLander, Angelika
dc.contributor.authorKurth, Andreas
dc.contributor.authorOrusa, Riccardo
dc.contributor.authorBertolotti, Luigi
dc.contributor.authorMandola, Maria Lucia
dc.date.accessioned2018-05-07T20:56:47Z
dc.date.available2018-05-07T20:56:47Z
dc.date.created2018-01-30
dc.date.issued2017-12-22none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rehZJIzvizps/PDF/22Fx7xaCBmmmY.pdf
dc.identifier.urihttp://edoc.rki.de/176904/2938
dc.description.abstractBackground: Bat-borne virus surveillance is necessary for determining inter-species transmission risks and is important due to the wide-range of bat species which may harbour potential pathogens. This study aimed to monitor coronaviruses (CoVs) and paramyxoviruses (PMVs) in bats roosting in northwest Italian regions. Our investigation was focused on CoVs and PMVs due to their proven ability to switch host and their zoonotic potential. Here we provide the phylogenetic characterization of the highly conserved polymerase gene fragments. Results: Family-wide PCR screenings were used to test 302 bats belonging to 19 different bat species. Thirty-eight animals from 12 locations were confirmed as PCR positive, with an overall detection rate of 12.6% [95% CI: 9.3–16.8]. CoV RNA was found in 36 bats belonging to eight species, while PMV RNA in three Pipistrellus spp. Phylogenetic characterization have been obtained for 15 alpha- CoVs, 5 beta-CoVs and three PMVs; moreover one P. pipistrellus resulted co-infected with both CoV and PMV. A divergent alpha-CoV clade from Myotis nattereri SpA is also described. The compact cluster of beta-CoVs from R. ferrumequinum roosts expands the current viral sequence database, specifically for this species in Europe. To our knowledge this is the first report of CoVs in Plecotus auritus and M. oxygnathus, and of PMVs in P. kuhlii. Conclusions: This study identified alpha and beta-CoVs in new bat species and in previously unsurveyed Italian regions. To our knowledge this represents the first and unique report of PMVs in Italy. The 23 new bat genetic sequences presented will expand the current molecular bat-borne virus databases. Considering the amount of novel bat-borne PMVs associated with the emergence of zoonotic infections in animals and humans in the last years, the definition of viral diversity within European bat species is needed. Performing surveillance studies within a specific geographic area can provide awareness of viral burden where bats roost in close proximity to spillover hosts, and form the basis for the appropriate control measures against potential threats for public health and optimal management of bats and their habitats.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectSurveillanceeng
dc.subjectGenetic characterizationeng
dc.subjectBat-borne viruseseng
dc.subjectCoronaviruseng
dc.subjectEmerging viruseseng
dc.subjectParamyxoviruseng
dc.subject.ddc610 Medizin
dc.titleCoronavirus and paramyxovirus in bats from Northwest Italy
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10056959
dc.identifier.doi10.1186/s12917-017-1307-x
dc.identifier.doihttp://dx.doi.org/10.25646/2863
local.edoc.container-titleBMC Veterinary Research
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://bmcvetres.biomedcentral.com/articles/10.1186/s12917-017-1307-x
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume13
local.edoc.container-issue396
local.edoc.container-year2017

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