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2018-03-22Zeitschriftenartikel DOI: 10.3390/v10040142
Rapid Viral Diagnosis of Orthopoxviruses by Electron Microscopy: Optional or a Must?
dc.contributor.authorGelderblom, Hans R.
dc.contributor.authorMadeley, Dick
dc.date.accessioned2018-05-07T21:18:46Z
dc.date.available2018-05-07T21:18:46Z
dc.date.created2018-04-20
dc.date.issued2018-03-22none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reGD5vyicy4hA/PDF/22sWOJAC62GjY.pdf
dc.identifier.urihttp://edoc.rki.de/176904/3056
dc.description.abstractDiagnostic electron microscopy (DEM) was an essential component of viral diagnosis until the development of highly sensitive nucleic acid amplification techniques (NAT). The simple negative staining technique of DEM was applied widely to smallpox diagnosis until the world-wide eradication of the human-specific pathogen in 1980. Since then, the threat of smallpox re-emerging through laboratory escape, molecular manipulation, synthetic biology or bioterrorism has not totally disappeared and would be a major problem in an unvaccinated population. Other animal poxviruses may also emerge as human pathogens. With its rapid results (only a few minutes after arrival of the specimen), no requirement for specific reagents and its “open view”, DEM remains an important component of virus diagnosis, particularly because it can easily and reliably distinguish smallpox virus or any other member of the orthopoxvirus (OPV) genus from parapoxviruses (PPV) and the far more common and less serious herpesviruses (herpes simplex and varicella zoster). Preparation, enrichment, examination, internal standards and suitable organisations are discussed to make clear its continuing value as a diagnostic technique.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectnegative stainingeng
dc.subjectdiagnostic electron microscopy (DEM)eng
dc.subjectrapid viral diagnosiseng
dc.subjectfebrile vesicular rasheseng
dc.subjectskin lesionseng
dc.subjectorthopoxviruses (OPV)eng
dc.subjectparapoxviruses (PPV)eng
dc.subjectherpesviruses (HSVeng
dc.subjectVZV)eng
dc.subject.ddc610 Medizin
dc.titleRapid Viral Diagnosis of Orthopoxviruses by Electron Microscopy: Optional or a Must?
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10058633
dc.identifier.doi10.3390/v10040142
dc.identifier.doihttp://dx.doi.org/10.25646/2981
local.edoc.container-titleViruses
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.mdpi.com/1999-4915/10/4/142
local.edoc.container-publisher-nameMDPI
local.edoc.container-volume10
local.edoc.container-issue142
local.edoc.container-year2018

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