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2008-12-03Zeitschriftenartikel DOI: 10.1186/1471-2180-8-210
Detection of Balamuthia mandrillaris DNA by real-time PCR targeting the RNase P gene
dc.contributor.authorKiderlen, Albrecht
dc.contributor.authorRadam, Elke
dc.contributor.authorLewin, Astrid
dc.date.accessioned2018-05-07T13:05:30Z
dc.date.available2018-05-07T13:05:30Z
dc.date.created2009-03-26
dc.date.issued2008-12-03none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rexBaW0zIAA/PDF/21AfOY402W9XU.pdf
dc.identifier.urihttp://edoc.rki.de/176904/385
dc.description.abstractBackground: The free-living amoeba Balamuthia mandrillaris may cause fatal encephalitis both in immunocompromised and in – apparently – immunocompetent humans and other mammalian species. Rapid, specific, sensitive, and reliable detection requiring little pathogen-specific expertise is an absolute prerequisite for a successful therapy and a welcome tool for both experimental and epidemiological research. Results: A real-time polymerase chain reaction assay using TaqMan® probes (real-time PCR) was established specifically targeting the RNase P gene of B. mandrillaris amoebae. The assay detected at least 2 (down to 0.5) genomes of B. mandrillaris grown in axenic culture. It did not react with DNA from closely related Acanthamoeba (3 species), nor with DNA from Toxoplasma gondii, Leishmania major, Pneumocystis murina, Mycobacterium bovis (BCG), human brain, various mouse organs, or from human and murine cell lines. The assay efficiently detected B. mandrillaris DNA in spiked cell cultures, spiked murine organ homogenates, B. mandrillaris-infected mice, and CNS tissue-DNA preparations from 2 patients with proven cerebral balamuthiasis. This novel primer set was successfully combined with a published set that targets the B. mandrillaris 18S rRNA gene in a duplex real-time PCR assay to ensure maximum specificity and as a precaution against false negative results. Conclusion: A real-time PCR assay for B. mandrillaris amoebae is presented, that is highly specific, sensitive, and reliable and thus suited both for diagnosis and for research.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut; Robert Koch-Institut, Infektionskrankheiten / Erreger
dc.rightsCreative Commons Namensnennung 3.0
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectAmebiasis/parasitologyeng
dc.subjectAmoeba/enzymologyeng
dc.subjectAmoeba/geneticseng
dc.subjectAmoeba/isolation & purificationeng
dc.subjectAnimalseng
dc.subjectDNAeng
dc.subjectProtozoan/geneticseng
dc.subjectRibosomal/geneticseng
dc.subjectHumanseng
dc.subjectPolymerase Chain Reaction/methodseng
dc.subjectProtozoan Proteins/geneticseng
dc.subjectProtozoan Proteins/metabolismeng
dc.subjectRNAeng
dc.subjectRibosomaleng
dc.subject18S/geneticseng
dc.subjectRibonuclease P/geneticseng
dc.subjectRibonuclease P/metabolismeng
dc.subjectSensitivity and Specificityeng
dc.subject.ddc610 Medizin
dc.titleDetection of Balamuthia mandrillaris DNA by real-time PCR targeting the RNase P gene
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10043
dc.identifier.doi10.1186/1471-2180-8-210
dc.identifier.doihttp://dx.doi.org/10.25646/310
local.edoc.container-titleBMC Microbiology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.biomedcentral.com/1471-2180/8/210
local.edoc.container-publisher-nameBioMed Central
local.edoc.container-volume8
local.edoc.container-issue210
local.edoc.container-year2008

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