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2009-06-21Zeitschriftenartikel DOI: 10.1186/1742-4690-6-60
Role of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication-deficient viral vector
dc.contributor.authorFalkensammer, Barbara
dc.contributor.authorRubner, Barbara
dc.contributor.authorHiltgartner, Alexander
dc.contributor.authorWilflingseder, Doris
dc.contributor.authorStahl-Hennig, Christiane
dc.contributor.authorKuate, Seraphin
dc.contributor.authorUeberla, Klaus
dc.contributor.authorNorley, Stephen
dc.contributor.authorStrasak, Alexander
dc.contributor.authorRacz, Paul
dc.contributor.authorStoiber, Heribert
dc.date.accessioned2018-05-07T13:16:34Z
dc.date.available2018-05-07T13:16:34Z
dc.date.created2009-09-01
dc.date.issued2009-06-21none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/revRET1jROuNY/PDF/29nXpis6P4Es.pdf
dc.identifier.urihttp://edoc.rki.de/176904/444
dc.description.abstractBackground: We investigated the interplay between complement and antibodies upon priming with single-cycle replicating viral vectors (SCIV) encoding SIV antigens combined with Adeno5-SIV or SCIV pseudotyped with murine leukemia virus envelope boosting strategies. The vaccine was applied via spray-immunization to the tonsils of rhesus macaques and compared with systemic regimens. Results: Independent of the application regimen or route, viral loads were significantly reduced after challenge with SIVmac239 (p < 0.03) compared to controls. Considerable amounts of neutralizing antibodies were induced in systemic immunized monkeys. Most of the sera harvested during peak viremia exhibited a trend with an inverse correlation between complement C3-deposition on viral particles and plasma viral load within the different vaccination groups. In contrast, the amount of the observed complement-mediated lysis did not correlate with the reduction of SIV titres. Conclusion: The heterologous prime-boost strategy with replication-deficient viral vectors administered exclusively via the tonsils did not induce any neutralizing antibodies before challenge. However, after challenge, comparable SIV-specific humoral immune responses were observed in all vaccinated animals. Immunization with single cycle immunodeficiency viruses mounts humoral immune responses comparable to live-attenuated immunodeficiency virus vaccines.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectAnimalseng
dc.subjectAntibodieseng
dc.subjectViral/immunologyeng
dc.subjectViral/bloodeng
dc.subjectAdenoviruseseng
dc.subjectHuman/geneticseng
dc.subjectAerosolseng
dc.subjectComplement System Proteins/immunologyeng
dc.subjectGenetic Vectorseng
dc.subjectLeukemia Viruseng
dc.subjectMurine/geneticseng
dc.subjectMacaca mulattaeng
dc.subjectNeutralization Testseng
dc.subjectPalatine Tonsil/virologyeng
dc.subjectSAIDS Vaccines/immunologyeng
dc.subjectSimian Acquired Immunodeficiency Syndrome/prevention & controleng
dc.subjectSimian immunodeficiency virus/geneticseng
dc.subjectSimian immunodeficiency virus/immunologyeng
dc.subjectViral Loadeng
dc.subject.ddc610 Medizin
dc.titleRole of complement and antibodies in controlling infection with pathogenic simian immunodeficiency virus (SIV) in macaques vaccinated with replication-deficient viral vector
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-1001371
dc.identifier.doi10.1186/1742-4690-6-60
dc.identifier.doihttp://dx.doi.org/10.25646/369
local.edoc.container-titleRetrovirology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.retrovirology.com/content/pdf/1742-4690-6-60.pdf
local.edoc.container-publisher-nameBiomedcentral
local.edoc.container-volume6
local.edoc.container-issue60
local.edoc.container-year2009

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