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2009-05-14Zeitschriftenartikel DOI: 10.1186/1743-422X-6-58
Detection of hepatitis E virus in wild boars of rural and urban regions in Germany and whole genome characterization of an endemic strain
dc.contributor.authorSchielke, Anika
dc.contributor.authorSachs, Katja
dc.contributor.authorLierz, Michael
dc.contributor.authorAppel, Bernd
dc.contributor.authorJansen, Andreas
dc.contributor.authorJohne, Reimar
dc.date.accessioned2018-05-07T13:23:49Z
dc.date.available2018-05-07T13:23:49Z
dc.date.created2009-11-25
dc.date.issued2009-05-14none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reUnaGfMdyQuM/PDF/25r6HfZMuD6O.pdf
dc.identifier.urihttp://edoc.rki.de/176904/483
dc.description.abstractBackground: Hepatitis E is an increasingly diagnosed human disease in Central Europe. Besides domestic pigs, in which hepatitis E virus (HEV) infection is highly prevalent, wild boars have been identified as a possible source of human infection. In order to assess the distribution of HEV in the wild boar population of Germany, we tested liver samples originating from different geographical regions for the presence of the HEV genome and compared the detected sequences to animal and human HEV strains. Results: A total of 148 wild boar liver samples were tested using real-time RT-PCR resulting in an average HEV detection rate of 14.9% (95% CI 9.6–21.6). HEV was detected in all age classes and all geographical regions. However, the prevalence of HEV infection was significantly higher in rural as compared to urban regions (p < 0.001). Sequencing of the PCR products indicated a high degree of heterogenicity of the detected viruses within genotype 3 and a grouping according to their geographical origin. The whole genome sequence of an HEV isolate (wbGER27) detected in many wild boars in the federal state of Brandenburg was determined. It belongs to genotype 3i and shows 97.9% nucleotide sequence identity to a partial sequence derived from a human hepatitis E patient from Germany. Conclusion: The results indicate that wild boars have to be considered as a reservoir for HEV in Germany and that a risk of HEV transmission to humans is present in rural as well as urban regions.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut; Robert Koch-Institut, Infektionsepidemiologie
dc.subjectAnimalseng
dc.subjectDNAeng
dc.subjectRNAeng
dc.subjectGermanyeng
dc.subjectGeographyeng
dc.subjectPrevalenceeng
dc.subjectMolecular Sequence Dataeng
dc.subjectSequence Analysiseng
dc.subjectPhylogenyeng
dc.subjectGenotypeeng
dc.subjectCluster Analysiseng
dc.subjectEndemic Diseases/veterinaryeng
dc.subjectGenomeeng
dc.subjectViraleng
dc.subjectHepatitis E/epidemiologyeng
dc.subjectHepatitis E/veterinaryeng
dc.subjectHepatitis E/virologyeng
dc.subjectHepatitis E virus/isolation & purificationeng
dc.subjectLiver/virologyeng
dc.subjectPolymorphismeng
dc.subjectGeneticeng
dc.subjectViral/geneticseng
dc.subjectSequence Homologyeng
dc.subjectNucleic Acideng
dc.subjectSus scrofa/virologyeng
dc.subjectSwine Diseases/epidemiologyeng
dc.subjectSwine Diseases/virologyeng
dc.subject.ddc610 Medizin
dc.titleDetection of hepatitis E virus in wild boars of rural and urban regions in Germany and whole genome characterization of an endemic strain
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-1002948
dc.identifier.doi10.1186/1743-422X-6-58
dc.identifier.doihttp://dx.doi.org/10.25646/408
local.edoc.container-titleVirology Journal
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.virologyj.com/content/6/1/58
local.edoc.container-publisher-nameBioMed Central
local.edoc.container-volume6
local.edoc.container-issue58
local.edoc.container-year2009

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