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2009-11-26Zeitschriftenartikel DOI: 10.1186/1743-422X-6-210
High genotypic diversity and a novel variant of human cytomegalovirus revealed by combined UL33/UL55 genotyping with broad-range PCR
dc.contributor.authorDeckers, Merlin
dc.contributor.authorHofmann, Jörg
dc.contributor.authorKreuzer, Karl-Anton
dc.contributor.authorReinhard, Henrike
dc.contributor.authorEdubio, Abigail
dc.contributor.authorHengel, Hartmut
dc.contributor.authorVoigt, Sebastian
dc.contributor.authorEhlers, Bernhard
dc.date.accessioned2018-05-07T13:27:21Z
dc.date.available2018-05-07T13:27:21Z
dc.date.created2009-12-07
dc.date.issued2009-11-26none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/regZ53HyXEDKU/PDF/23iUP6DuxWpTU.pdf
dc.identifier.urihttp://edoc.rki.de/176904/502
dc.description.abstractThe known strains of human cytomegalovirus (HCMV) represent genotypic variants of a single species, and HCMV genotypic variability has been studied in order to reveal correlations between different disease patterns and the presence of certain HCMV genotypes, either as single or as multiple infections. The methods used for the detection of HCMV genotypes have not always been sophisticated enough to achieve complete comprehensiveness, mainly because only one genotype is usually detected in a certain specimen, due to primer specificity and genome copy number. To improve detection of variant HCMV genotypes in mixed infections, we developed PCR assays with degenerate primers targeting two variable HCMV genes, glycoprotein B (gB, UL55) and the G-protein-coupled receptor gene UL33. Primers were designed to bind conserved sites in the genomes of HCMV variants and great ape CMVs. To analyse if samples contained one or more HCMV genotypic variants, PCR assays were supplemented with oligonucleotides containing locked nucleic acids. This broad-range PCR methodology and subsequent sequence analysis detected all gB/UL55 and UL33 genotypic variants known to date in primary clinical specimens, but also revealed that many samples contained genotype mixtures. Importantly, a novel UL33 genotypic variant could be discovered in several specimens, and one HCMV isolate was plaque-purified containing the novel UL33 genotype and a so far undescribed variant of gB.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Stammzellen
dc.subjectAnimalseng
dc.subjectPreschooleng
dc.subjectHumanseng
dc.subjectPhylogenyeng
dc.subjectSequence Homologyeng
dc.subjectAmino Acideng
dc.subjectCluster Analysiseng
dc.subjectChildeng
dc.subjectSensitivity and Specificityeng
dc.subjectViral Proteins/geneticseng
dc.subjectGenetic Variationeng
dc.subjectPolymerase Chain Reaction/methodseng
dc.subjectDNA Primers/geneticseng
dc.subjectCytomegalovirus/geneticseng
dc.subjectCytomegalovirus/isolation & purificationeng
dc.subjectCytomegalovirus Infections/virologyeng
dc.subjectHominidaeeng
dc.subjectReceptorseng
dc.subjectChemokine/geneticseng
dc.subjectViral Envelope Proteins/geneticseng
dc.subject.ddc610 Medizin
dc.titleHigh genotypic diversity and a novel variant of human cytomegalovirus revealed by combined UL33/UL55 genotyping with broad-range PCR
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-1003207
dc.identifier.doi10.1186/1743-422X-6-210
dc.identifier.doihttp://dx.doi.org/10.25646/427
local.edoc.container-titleVirology Journal
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.virologyj.com/content/6/1/210
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume6
local.edoc.container-issue210
local.edoc.container-year2009

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