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2015-03-04Bachelorarbeit DOI: 10.25646/5342
Detection of DNA intermediates of the reverse transcription during the infection process of the human endogenous retrovirus-K (HML-2)
dc.contributor.authorVolkwein, Alexander
dc.date.accessioned2018-05-08T04:42:50Z
dc.date.available2018-05-08T04:42:50Z
dc.date.created2015-11-27
dc.date.issued2015-03-04none
dc.identifier.otherhttp://edoc.rki.de/documents/dissertationen/volkwein-alexander-2015-03-04/PDF/volkwein.pdf
dc.identifier.urihttp://edoc.rki.de/176904/5417
dc.description.abstractEndogenous retroviruses form part of the genome of many vertebrates. In humans they account for approximately 8 % of the entire genetic information. Along evolution the proviral sequences have been mutated until nowadays there is no known HERV able to express infectious particles. However the youngest and probably therefore the less mutated family, HERV-K (HML-2), preserves proviruses able to produce functional proteins. Although it has been shown that not only the glycoproteins responsible for mediating entry have been mutated, but also integration of the viral genes in the host genome will not be properly accomplished. In order to determine potential inhibited stages of this process, HERV-K pseudotyped viral particles able to mediate penetration of the cell membrane were successfully produced and monitored during reverse transcription on their course towards integration. For this purpose several set of primers were designed, that bind specific to different stages along the reverse transcription process. Using these primers reverse transcribed DNA could be detected. Consequently, analysis of these characteristics steps could ideally help to detect at some point of significant less cDNA, which may permit to infer the phase where inhibition takes place.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.titleDetection of DNA intermediates of the reverse transcription during the infection process of the human endogenous retrovirus-K (HML-2)
dc.typebachelorThesis
dc.identifier.urnurn:nbn:de:0257-10041709
dc.identifier.doihttp://dx.doi.org/10.25646/5342
dc.date.accepted2015-03-04
local.edoc.pages38
local.edoc.type-nameBachelorarbeit
local.edoc.universityFreie Universität Berlin

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