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2018-04-19Zeitschriftenartikel DOI: 10.25646/5676
Optimisation of quantitative miRNA panels to consolidate the diagnostic surveillance of HBV-related hepatocellular carcinoma
dc.contributor.authorTat Trung, Ngo
dc.contributor.authorDuong, Dang Chieu
dc.contributor.authorTong, Hoang Van
dc.contributor.authorHien, Tran Thi Thu
dc.contributor.authorHoan, Phan Quoc
dc.contributor.authorBang, Mai Hong
dc.contributor.authorBinh, Mai Thanh
dc.contributor.authorKy, Thai Doan
dc.contributor.authorTung, Nguyen Lam
dc.contributor.authorThinh, Nguyen Tien
dc.contributor.authorSang, Vu Viet
dc.contributor.authorThao, Le Thi Phuong
dc.contributor.authorBock, Thomas
dc.date.accessioned2018-09-12T08:13:06Z
dc.date.available2018-09-12T08:13:06Z
dc.date.issued2018-04-19none
dc.identifier.other10.1371/journal.pone.0196081
dc.identifier.urihttp://edoc.rki.de/176904/5741
dc.description.abstractBackground: Circulating microRNAs (miRNA) are biomarkers for several neoplastic diseases, including hepatocellular carcinoma (HCC). We performed a literature search, followed by experimental screening and validation in order to establish a miRNA panel in combination with the assessment of alpha-fetoprotein (AFP) levels and to evaluate its performance in HCC diagnostics. Methods: Expression of miRNAs was quantified by quantitative PCR (qPCR) in 406 serum samples from 118 Vietnamese patients with hepatitis B (HBV)-related HCC, 69 patients with HBV-related liver cirrhosis (LC), 100 chronic hepatitis B (CHB) patients and 119 healthy controls (HC). Results: Three miRNAs (mir-21, mir-122, mir-192) were expressed differentially among the studied subgroups and positively correlated with AFP levels. The individual miRNAs mir-21, mir-122, mir192 or the triplex miRNA panel showed high diagnostic accuracy for HCC (HCC vs. CHB, AUC = 0.906; HCC vs. CHB+LC, AUC = 0.81; HCC vs. CHB+LC+HC, AUC = 0.854). When AFP levels were ≤20ng/ml, the triplex miRNA panel still was accurate in distinguishing HCC from the other conditions (CHB, AUC = 0.922; CHB+LC, AUC = 0.836; CHB+LC+HC, AUC = 0.862). When AFP levels were used in combination with the triplex miRNA panel, the diagnostic performance was significantly improved in discriminating HCC from the other groups (LC, AUC = 0.887; CHB, AUC = 0.948; CHB+LC, AUC = 0.887). Conclusions: The three miRNAs mir-21, mir-122, mir-192, together with AFP, are biomarkers that may be applied to improve diagnostics of HCC in HBV patients, especially in HBV-related LC patients with normal AFP levels or HCC patients with small tumor sizes.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleOptimisation of quantitative miRNA panels to consolidate the diagnostic surveillance of HBV-related hepatocellular carcinomanone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/5741-1
dc.identifier.doihttp://dx.doi.org/10.25646/5676
dc.type.versionpublishedVersionnone
local.edoc.container-titlePLoS ONEnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://journals.plos.org/plosone/article?id=10.1371/journal.pone.0196081none
local.edoc.container-publisher-namePublic Library of Sciencenone
local.edoc.container-volume13none
local.edoc.container-issue4none
local.edoc.container-reportyear2018none
local.edoc.container-firstpage1none
local.edoc.container-lastpage17none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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