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2018-06-14Zeitschriftenartikel DOI: 10.25646/5697
Smear Microscopy for Diagnosis of Pulmonary Tuberculosis in Eastern Sudan
dc.contributor.authorShuaib, Yassir A.
dc.contributor.authorKhalil, Eltahir A. G.
dc.contributor.authorSchaible, Ulrich E.
dc.contributor.authorWieler, Lothar H.
dc.contributor.authorBakheit, Mohammed A. M.
dc.contributor.authorMohamed-Noor, Saad E.
dc.contributor.authorAbdalla, Mohamed A.
dc.contributor.authorHomolka, Susanne
dc.contributor.authorAndres, Sönke
dc.contributor.authorHillemann, Doris
dc.contributor.authorLonnroth, Knut
dc.contributor.authorRichter, Elvira
dc.contributor.authorNiemann, Stefan
dc.contributor.authorKranzer, Katharina
dc.date.accessioned2018-09-18T13:48:03Z
dc.date.available2018-09-18T13:48:03Z
dc.date.issued2018-06-14none
dc.identifier.other10.1155/2018/8038137
dc.identifier.urihttp://edoc.rki.de/176904/5760
dc.description.abstractBackground. In Sudan, tuberculosis diagnosis largely relies on clinical symptoms and smear microscopy as in many other low- and middle-income countries. The aim of this study was to investigate the positive predictive value of a positive sputum smear in patients investigated for pulmonary tuberculosis in Eastern Sudan. Methods. Two sputum samples from patients presenting with symptoms suggestive of tuberculosis were investigated using direct Ziehl-Neelsen (ZN) staining and light microscopy between June to October 2014 and January to July 2016. If one of the samples was smear positive, both samples were pooled, stored at −20°C, and sent to the National Reference Laboratory (NRL), Germany. Following decontamination, samples underwent repeat microscopy and culture. Culture negative/contaminated samples were investigated using polymerase chain reaction (PCR). Results. A total of 383 samples were investigated. Repeat microscopy categorized 123 (32.1%) as negative, among which 31 were culture positive. This increased to 80 when PCR and culture results were considered together. A total of 196 samples were culture positive, of which 171 (87.3%), 14 (7.1%), and 11 (5.6%) were M. tuberculosis, M. intracellulare, and mixed species. Overall, 15.6% (57/365) of the samples had no evidence of M. tuberculosis, resulting in a positive predictive value of 84.4%. Conclusions. There was a discordance between the results of smear microscopy performed at local laboratories in the Sudan and at the NRL, Germany; besides, a considerable number of samples had no evidence of M. tuberculosis. Improved quality control for smear microscopy and more specific diagnostics are crucial to avoid possible overtreatment.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleSmear Microscopy for Diagnosis of Pulmonary Tuberculosis in Eastern Sudannone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/5760-7
dc.identifier.doihttp://dx.doi.org/10.25646/5697
dc.type.versionpublishedVersionnone
local.edoc.container-titleTuberculosis Research and Treatmentnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.hindawi.com/journals/trt/2018/8038137/none
local.edoc.container-publisher-nameHindawi Publishing Corporationnone
local.edoc.container-volume2018none
local.edoc.container-reportyear2018none
local.edoc.container-firstpage1none
local.edoc.container-lastpage8none
local.edoc.rki-departmentInstitutsleitungnone
dc.description.versionPeer Reviewednone

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