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2018-08-15Zeitschriftenartikel DOI: 10.25646/5725
Bacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogens
dc.contributor.authorKunstmann, Sonja
dc.contributor.authorScheidt, Tom
dc.contributor.authorBuchwald, Saskia
dc.contributor.authorHelm, Alexandra
dc.contributor.authorMulard, Laurence A.
dc.contributor.authorFruth, Angelika
dc.contributor.authorBarbirz, Stefanie
dc.date.accessioned2018-10-01T09:51:40Z
dc.date.available2018-10-01T09:51:40Z
dc.date.issued2018-08-15none
dc.identifier.other10.3390/v10080431
dc.identifier.urihttp://edoc.rki.de/176904/5786
dc.description.abstractBacteriophage research is gaining more importance due to increasing antibiotic resistance. However, for treatment with bacteriophages, diagnostics have to be improved. Bacteriophages carry adhesion proteins, which bind to the bacterial cell surface, for example tailspike proteins (TSP) for specific recognition of bacterial O-antigen polysaccharide. TSP are highly stable proteins and thus might be suitable components for the integration into diagnostic tools. We used the TSP of bacteriophage Sf6 to establish two applications for detecting Shigella flexneri (S. flexneri), a highly contagious pathogen causing dysentery. We found that Sf6TSP not only bound O-antigen of S. flexneri serotype Y, but also the glucosylated O-antigen of serotype 2a. Moreover, mass spectrometry glycan analyses showed that Sf6TSP tolerated various O-acetyl modifications on these O-antigens. We established a microtiter plate-based ELISA like tailspike adsorption assay (ELITA) using a Strep-tag®II modified Sf6TSP. As sensitive screening alternative we produced a fluorescently labeled Sf6TSP via coupling to an environment sensitive dye. Binding of this probe to the S. flexneri O-antigen Y elicited a fluorescence intensity increase of 80% with an emission maximum in the visible light range. The Sf6TSP probes thus offer a promising route to a highly specific and sensitive bacteriophage TSP-based Shigella detection system.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectShigella flexnerieng
dc.subjectbacteriophageeng
dc.subjecttailspike proteinseng
dc.subjectO-antigeneng
dc.subjectserotypingeng
dc.subjectmicrotiter plate assayeng
dc.subjectfluorescence sensoreng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleBacteriophage Sf6 Tailspike Protein for Detection of Shigella flexneri Pathogensnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/5786-2
dc.identifier.doihttp://dx.doi.org/10.25646/5725
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirusesnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/1999-4915/10/8/431none
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume10none
local.edoc.container-issue8none
local.edoc.container-reportyear2018none
local.edoc.container-firstpage1none
local.edoc.container-lastpage18none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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