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2018-10-24Zeitschriftenartikel DOI: 10.25646/5835
Equine Methicillin-Resistant Sequence Type 398 Staphylococcus aureus (MRSA) Harbor Mobile Genetic Elements Promoting Host Adaptation
dc.contributor.authorWalther, Birgit
dc.contributor.authorKlein, Katja-Sophia
dc.contributor.authorBarton, Ann-Kristin
dc.contributor.authorSemmler, Torsten
dc.contributor.authorHuber, Charlotte
dc.contributor.authorMerle, Roswitha
dc.contributor.authorTedin, Karsten
dc.contributor.authorMitrach, Franziska
dc.contributor.authorLübke-Becker, Antina
dc.contributor.authorGehlen, Heidrun
dc.date.accessioned2018-12-17T11:24:12Z
dc.date.available2018-12-17T11:24:12Z
dc.date.issued2018-10-24none
dc.identifier.other10.3389/fmicb.2018.02516
dc.identifier.urihttp://edoc.rki.de/176904/5884
dc.description.abstractContinuing introduction of multi-drug resistant, zoonotic pathogens such as methicillin-resistant Staphylococcus aureus (MRSA) in horse clinics challenges the biosafety of employees and animal patients. This study was aimed to determine the occurrence of mobile genetic elements facilitating survival in the early stages of invasive infection in different host species, including humans and horses, in MRSA carried by equine patients admitted to a large horse clinic. A total of 341 equine patients were investigated for carriage of MRSA by hygiene screening directly at hospital admission. MRSA were further investigated by antimicrobial susceptibility testing, whole-genome sequencing and genomic composition, including virulence factors involved in immune evasion and host adaption. From a total of 340 validated specimens from equine nostrils, 3.5% yielded positive results for MRSA. All MRSA were found to be closely related belonging to sequence type (ST) 398_t011 with up to four additional antimicrobial resistances. All MRSA harbored a specific Staphylococcal Pathogenicity Island (SaPIbov5) involved in facilitating survival in ruminant and equine plasma. Moreover, a β-hemolysin (hlb) converting ΦSa3 phage encoding the human-specific Immune Evasion Cluster (IEC) was present in 72% of the isolates. An equid-specific leukotoxin encoded by a further temperate phage (Saeq1) was only rarely detected (22%). Despite the absence of β-hemolysin production for all IEC-positive ST398, a prominent hemolysis zone was demonstrable on sheep blood agar. Thus, IEC might remain undetected among the ST398 lineage, since the presence of IEC is commonly associated with reduction of hemolysis in S. aureus belonging to other genetic backgrounds. Here we describe MRSA-ST398 harboring different mobile genetic elements encoding variants of immune evasion factors and toxins previously shown to contribute to S. aureus invasive diseases in specific host species or ecologic niches. We suggest these combinations contribute to the adaptation of MRSA belonging to ST398 with respect to epidemic spread across different habitats and hosts, and may therefore confer a host “generalist” phenotype.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleEquine Methicillin-Resistant Sequence Type 398 Staphylococcus aureus (MRSA) Harbor Mobile Genetic Elements Promoting Host Adaptationnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/5884-6
dc.identifier.doihttp://dx.doi.org/10.25646/5835
dc.type.versionpublishedVersionnone
local.edoc.container-titleFrontiers in Microbiologynone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.frontiersin.org/articles/10.3389/fmicb.2018.02516/fullnone
local.edoc.container-publisher-nameFrontiers Medianone
local.edoc.container-volume9none
local.edoc.container-issue2516none
local.edoc.container-reportyear2018none
local.edoc.container-firstpage1none
local.edoc.container-lastpage10none
local.edoc.rki-departmentProjektgruppen/Nachwuchsgruppennone
dc.description.versionPeer Reviewednone

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