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2019-01-16Zeitschriftenartikel DOI: 10.25646/5934
The cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymes
dc.contributor.authorCleverley, Robert M.
dc.contributor.authorRutter, Zoe J.
dc.contributor.authorRismondo, Jeanine
dc.contributor.authorCorona, Federico
dc.contributor.authorTsui, Ho-Ching Tiffany
dc.contributor.authorAlatawi, Fuad A.
dc.contributor.authorDaniel, Richard A.
dc.contributor.authorHalbedel, Sven
dc.contributor.authorMassidda, Orietta
dc.contributor.authorWinkler, Malcolm E.
dc.contributor.authorLewis, Richard J.
dc.date.accessioned2019-03-06T09:50:57Z
dc.date.available2019-03-06T09:50:57Z
dc.date.issued2019-01-16none
dc.identifier.other10.1038/s41467-018-08056-2
dc.identifier.urihttp://edoc.rki.de/176904/5969
dc.description.abstractBacterial growth and cell division requires precise spatiotemporal regulation of the synthesis and remodelling of the peptidoglycan layer that surrounds the cytoplasmic membrane. GpsB is a cytosolic protein that affects cell wall synthesis by binding cytoplasmic mini-domains of peptidoglycan synthases to ensure their correct subcellular localisation. Here, we describe critical structural features for the interaction of GpsB with peptidoglycan synthases from three bacterial species (Bacillus subtilis, Listeria monocytogenes and Streptococcus pneumoniae) and suggest their importance for cell wall growth and viability in L. monocytogenes and S. pneumoniae. We use these structural motifs to identify novel partners of GpsB in B. subtilis and extend the members of the GpsB interactome in all three bacterial species. Our results support that GpsB functions as an adaptor protein that mediates the interaction between membrane proteins, scaffolding proteins, signalling proteins and enzymes to generate larger protein complexes at specific sites in a bacterial cell cycle-dependent manner.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectBacterial structural biologyeng
dc.subjectCellular microbiologyeng
dc.subjectX-ray crystallographyeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleThe cell cycle regulator GpsB functions as cytosolic adaptor for multiple cell wall enzymesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/5969-7
dc.identifier.doihttp://dx.doi.org/10.25646/5934
dc.type.versionpublishedVersionnone
local.edoc.container-titleNature Communicationsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/articles/s41467-018-08056-2#article-infonone
local.edoc.container-publisher-nameNature Publishing Groupnone
local.edoc.container-volume2019none
local.edoc.container-issue10none
local.edoc.container-reportyear2019none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage17none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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