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2019-03-06Zeitschriftenartikel DOI: 10.25646/6037
Diagnosing Zika virus infection against a background of other flaviviruses: Studies in high resolution serological analysis
dc.contributor.authorHansen, Sören
dc.contributor.authorHotop, Sven-Kevin
dc.contributor.authorFaye, Oumar
dc.contributor.authorNdiaye, Oumar
dc.contributor.authorBöhlken-Fascher, Susanne
dc.contributor.authorPessôa, Rodrigo
dc.contributor.authorHufert, Frank
dc.contributor.authorStahl-Hennig, Christiane
dc.contributor.authorFrank, Roland
dc.contributor.authorCzerny, Claus-Peter
dc.contributor.authorSchmidt-Chanasit, Jonas
dc.contributor.authorSanabani, Sabri S.
dc.contributor.authorSall, Amadou A.
dc.contributor.authorNiedrig, Matthias
dc.contributor.authorBrönstrup, Mark
dc.contributor.authorFritz, Hans-Joachim
dc.contributor.authorAbd El Wahed, Ahmed
dc.date.accessioned2019-03-29T10:16:40Z
dc.date.available2019-03-29T10:16:40Z
dc.date.issued2019-03-06none
dc.identifier.other10.1038/s41598-019-40224-2
dc.identifier.urihttp://edoc.rki.de/176904/6072
dc.description.abstractZika virus (ZIKV) is a mosquito-borne flavivirus. Homologous proteins of different flaviviruses display high degrees of sequence identity, especially within subgroups. This leads to extensive immunological cross-reactivity and corresponding problems for developing a ZIKV-specific serological assay. In this study, peptide microarrays were employed to identify individual ZIKV antibody targets with promise in differential diagnosis. A total of 1643 overlapping oligopeptides were synthesized and printed onto glass slides. Together, they encompass the full amino acid sequences of ZIKV proteomes of African, Brazilian, USA, and French Polynesian origins. The resulting ZIKV scanning microarray chips were used to screen three pools of sera from recent Zika outbreaks in Senegal and Cape Verde, in Brazil, and from overseas travelers returning to the EU. Together with a mixed pool of well characterized, archived sera of patients suffering from infections by dengue, yellow fever, tick-borne encephalitis, and West Nile viruses, a total of 42 sera went into the study. Sixty-eight antibody target regions were identified. Most of which were hitherto unknown. Alignments and sequence comparisons revealed 13 of which could be classified as bona fide ZIKV-specific. These identified antibody target regions constitute a founding set of analytical tools for serological discrimination of ZIKV from other flaviviruses.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectDiagnosiseng
dc.subjectViral infectioneng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleDiagnosing Zika virus infection against a background of other flaviviruses: Studies in high resolution serological analysisnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6072-1
dc.identifier.doihttp://dx.doi.org/10.25646/6037
dc.type.versionpublishedVersionnone
local.edoc.container-titleScientific Reportsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/articles/s41598-019-40224-2#Abs1none
local.edoc.container-publisher-nameNature Publishing Groupnone
local.edoc.container-volume9none
local.edoc.container-issue3648none
local.edoc.container-reportyear2019none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage10none
local.edoc.rki-departmentInstitutsleitungnone
dc.description.versionPeer Reviewednone

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