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2019-01-16Zeitschriftenartikel DOI: 10.25646/6119
A Listeria monocytogenes ST2 clone lacking chitinase ChiB from an outbreak of non-invasive gastroenteritis
dc.contributor.authorHalbedel, Sven
dc.contributor.authorPrager, Rita
dc.contributor.authorKleta, Sylvia
dc.contributor.authorTrost, Eva
dc.contributor.authorNishanth, Gopala
dc.contributor.authorAlles, Georg
dc.contributor.authorHölzel, Christina
dc.contributor.authorSchlesiger, Friederike
dc.contributor.authorPietzka, Ariane
dc.contributor.authorSchlüter, Dirk
dc.contributor.authorFlieger, Antje
dc.contributor.authorBanerji, Sangeeta
dc.date.accessioned2019-04-26T11:38:20Z
dc.date.available2019-04-26T11:38:20Z
dc.date.issued2019-01-16none
dc.identifier.other10.1080/22221751.2018.1558960
dc.identifier.urihttp://edoc.rki.de/176904/6147
dc.description.abstractAn outbreak with a remarkable Listeria monocytogenes clone causing 163 cases of non-invasive listeriosis occurred in Germany in 2015. Core genome multi locus sequence typing grouped non-invasive outbreak isolates and isolates obtained from related food samples into a single cluster, but clearly separated genetically close isolates obtained from invasive listeriosis cases. A comparative genomic approach identified a premature stop codon in the chiB gene, encoding one of the two L. monocytogenes chitinases, which clustered with disease outcome. Correction of this premature stop codon in one representative gastroenteritis outbreak isolate restored chitinase production, but effects in infection experiments were not found. While the exact role of chitinases in virulence of L. monocytogenes is still not fully understood, our results now clearly show that ChiB-derived activity is not required to establish L. monocytogenes gastroenteritis in humans. This limits a possible role of ChiB in human listeriosis to later steps of the infection.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectMolecular surveillanceeng
dc.subjectchitineng
dc.subjectlisteriosiseng
dc.subjectinvasioneng
dc.subjectcore genome MLSTeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleA Listeria monocytogenes ST2 clone lacking chitinase ChiB from an outbreak of non-invasive gastroenteritisnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6147-8
dc.identifier.doihttp://dx.doi.org/10.25646/6119
dc.type.versionpublishedVersionnone
local.edoc.container-titleEmerging Microbes & Infectionsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.tandfonline.com/doi/full/10.1080/22221751.2018.1558960?scroll=top&needAccess=truenone
local.edoc.container-publisher-nameNature Publishing Groupnone
local.edoc.container-volume8none
local.edoc.container-issue1none
local.edoc.container-reportyear2019none
local.edoc.container-year2019none
local.edoc.container-firstpage17none
local.edoc.container-lastpage28none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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