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2019-10-10Zeitschriftenartikel DOI: 10.25646/6469
Novel Polyomaviruses in Mammals from Multiple Orders and Reassessment of Polyomavirus Evolution and Taxonomy
dc.contributor.authorEhlers, Bernhard
dc.contributor.authorAnoh, Augustin E.
dc.contributor.authorBen Salem, Nicole
dc.contributor.authorBroll, Sebastian
dc.contributor.authorCouacy-Hymann, Emmanuel
dc.contributor.authorFischer, Daniela
dc.contributor.authorGedvilaite, Alma
dc.contributor.authorIngenhütt, Nanina
dc.contributor.authorLiebmann, Sonja
dc.contributor.authorMartin, Maite
dc.contributor.authorMousson, Arsene
dc.contributor.authorMugisha, Lawrence
dc.contributor.authorMuyembe-Tamfum, Jean-Jacques
dc.contributor.authorPauly, Maude
dc.contributor.authorPeréz de Val, Bernat
dc.contributor.authorPreugschas, Hannah
dc.contributor.authorRichter, Dania
dc.contributor.authorSchubert, Grit
dc.contributor.authorSzentiks, Claudia A.
dc.contributor.authorTeichmann, Tamara
dc.contributor.authorWalter, Cornelia
dc.contributor.authorUlrich, Rainer G.
dc.contributor.authorWiersma, Lidewij
dc.contributor.authorLeendertz, Fabian H.
dc.contributor.authorCalvignac-Spencer, Sébastien
dc.date.accessioned2020-01-30T11:53:25Z
dc.date.available2020-01-30T11:53:25Z
dc.date.issued2019-10-10none
dc.identifier.other10.3390/v11100930
dc.identifier.urihttp://edoc.rki.de/176904/6472
dc.description.abstractAs the phylogenetic organization of mammalian polyomaviruses is complex and currently incompletely resolved, we aimed at a deeper insight into their evolution by identifying polyomaviruses in host orders and families that have either rarely or not been studied. Sixteen unknown and two known polyomaviruses were identified in animals that belong to 5 orders, 16 genera, and 16 species. From 11 novel polyomaviruses, full genomes could be determined. Splice sites were predicted for large and small T antigen (LTAg, STAg) coding sequences (CDS) and examined experimentally in transfected cell culture. In addition, splice sites of seven published polyomaviruses were analyzed. Based on these data, LTAg and STAg annotations were corrected for 10/86 and 74/86 published polyomaviruses, respectively. For 25 polyomaviruses, a spliced middle T CDS was observed or predicted. Splice sites that likely indicate expression of additional, alternative T antigens, were experimentally detected for six polyomaviruses. In contrast to all other mammalian polyomaviruses, three closely related cetartiodactyl polyomaviruses display two introns within their LTAg CDS. In addition, the VP2 of Glis glis (edible dormouse) polyomavirus 1 was observed to be encoded by a spliced transcript, a unique experimental finding within the Polyomaviridae family. Co-phylogenetic analyses based on LTAg CDS revealed a measurable signal of codivergence when considering all mammalian polyomaviruses, most likely driven by relatively recent codivergence events. Lineage duplication was the only other process whose influence on polyomavirus evolution was unambiguous. Finally, our analyses suggest that an update of the taxonomy of the family is required, including the creation of novel genera of mammalian and non-mammalian polyomaviruses.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.subjectpolyomaviruseng
dc.subjectgenomeeng
dc.subjectevolutioneng
dc.subjectT antigeneng
dc.subjectVP2eng
dc.subjectsplicingeng
dc.subjecttaxonomyeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleNovel Polyomaviruses in Mammals from Multiple Orders and Reassessment of Polyomavirus Evolution and Taxonomynone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6472-3
dc.identifier.doihttp://dx.doi.org/10.25646/6469
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirusesnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/1999-4915/11/10/930none
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume11none
local.edoc.container-issue10none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage27none
local.edoc.rki-departmentProjektgruppen/Nachwuchsgruppennone
dc.description.versionPeer Reviewednone

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