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2019-09-13Zeitschriftenartikel DOI: 10.25646/6546
Molecular Characterization of Equine Staphylococcus aureus Isolates Exhibiting Reduced Oxacillin Susceptibility
dc.contributor.authorScholtzek, Anissa D.
dc.contributor.authorHanke, Dennis
dc.contributor.authorWalther, Birgit
dc.contributor.authorEichhorn, Inga
dc.contributor.authorStöckle, Sabita D.
dc.contributor.authorKlein, Katja-Sophia
dc.contributor.authorGehlen, Heidrun
dc.contributor.authorLübke-Becker, Antina
dc.contributor.authorSchwarz, Stefan
dc.contributor.authorFeßler, Andrea T.
dc.date.accessioned2020-03-17T08:37:27Z
dc.date.available2020-03-17T08:37:27Z
dc.date.issued2019-09-13none
dc.identifier.other10.3390/toxins11090535
dc.identifier.urihttp://edoc.rki.de/176904/6515
dc.description.abstractThe detection of borderline oxacillin-resistant Staphylococcus aureus (BORSA) represents a challenge to both, veterinary and human laboratories. Between 2015 and 2017, 19 equine S. aureus with elevated minimal inhibitory concentrations for oxacillin were detected in routine diagnostics. The aim of this study was to characterize these isolates to identify factors possibly associated with the BORSA phenotype. All S. aureus were subjected to antimicrobial susceptibility testing and whole genome sequencing (WGS). A quantifiable β-lactamase activity assay was performed for a representative subset of 13 isolates. The WGS data analysis of the 19 BORSA isolates identified two different genomic lineages, sequence type (ST) 1 and ST1660. The core genome multilocus sequence typing (cgMLST) revealed a close relatedness of all isolates belonging to either ST1 or ST1660. The WGS analysis identified the resistance genes aadD, dfrG, tet(L), and/or blaZ and aacA-aphD. Phenotypic resistance to penicillins, aminoglycosides, tetracyclines, fluoroquinolones and sulfamethoxazole/trimethoprim was observed in the respective isolates. For the penicillin-binding proteins 1–4, amino acid substitutions were predicted using WGS data. Since neither transglycosylase nor transpeptidase domains were affected, these alterations might not explain the BORSA phenotype. Moreover, β-lactamase activity was found to be associated with an inducible blaZ gene. Lineage-specific differences regarding the expression profiles were noted.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectborderline oxacillin resistanceeng
dc.subjectBORSAeng
dc.subjectsusceptibility testingeng
dc.subjectMSSAeng
dc.subjectwhole genome sequencingeng
dc.subjectblaZeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleMolecular Characterization of Equine Staphylococcus aureus Isolates Exhibiting Reduced Oxacillin Susceptibilitynone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6515-2
dc.identifier.doihttp://dx.doi.org/10.25646/6546
dc.type.versionpublishedVersionnone
local.edoc.container-titleToxinsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/2072-6651/11/9/535none
local.edoc.container-publisher-nameMolecular Diversity Preservation International (MDPI)none
local.edoc.container-volume11none
local.edoc.container-issue9none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage20none
local.edoc.rki-departmentZentrum für Biologische Gefahren und Spezielle Pathogenenone
dc.description.versionPeer Reviewednone

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