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2019-12-29Zeitschriftenartikel DOI: 10.25646/6555
A Comprehensive Strategy for Screening for Xenotransplantation-Relevant Viruses in a Second Isolated Population of Göttingen Minipigs
dc.contributor.authorKrüger, Luise
dc.contributor.authorKristiansen, Yannick
dc.contributor.authorReuber, Emelie
dc.contributor.authorMöller, Lars
dc.contributor.authorLaue, Michael
dc.contributor.authorReimer, Christian
dc.contributor.authorDenner, Joachim
dc.date.accessioned2020-03-18T08:11:35Z
dc.date.available2020-03-18T08:11:35Z
dc.date.issued2019-12-29none
dc.identifier.other10.3390/v12010038
dc.identifier.urihttp://edoc.rki.de/176904/6523
dc.description.abstractXenotransplantation using pig tissues and organs is under development in order to alleviate the increasing shortage of human transplants. Since xenotransplantation may be associated with the transmission of porcine microorganisms to the human recipient, the donor pigs should be carefully analyzed, especially for the presence of potentially zoonotic viruses. Göttingen Minipigs (GöMP) are potential donors of islet cells for the treatment of diabetes. Despite the fact that all animals produced at Ellegaard Göttingen Minipigs A/S carry porcine endogenous retroviruses (PERVs) in their genome and that very few animals were infected with porcine cytomegalovirus (PCMV), hepatitis E virus (HEV) and porcine lymphotropic herpesvirus (PLHV), no transmission of these viruses was observed in a preclinical trial transplanting GöMP islet cells into cynomolgus monkeys. Using a new comprehensive strategy, we then analyzed an isolated subpopulation of Göttingen Minipigs which remained at the University of Göttingen. We concentrated on 11 xenotransplantation-relevant viruses and combined co-incubation assays with susceptible human target cells and molecular biological methods to evaluate the risk posed by PERV. All animals in Göttingen carry PERV-A, PERV-B, and PERV-C in their genome but they are not infected with PCMV, PLHV and HEV. The difference may be explained by selection of negative animals and/or de novo infection. The PERV copy number was established using ddPCR (93 copies) and a human-tropic PERV-A/C was found released from PBMCs of one animal with a high expression of PERV-C.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectporcine viruseseng
dc.subjectxenotransplantationeng
dc.subjectporcine endogenous retroviruses (PERV)eng
dc.subjectporcine cytomegalovirus (PCMV)eng
dc.subjecthepatitis E virus (HEV)eng
dc.subjectporcine lymphotropic herpesvirus (PLHV)eng
dc.subjectporcine circovirus (PCV)eng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleA Comprehensive Strategy for Screening for Xenotransplantation-Relevant Viruses in a Second Isolated Population of Göttingen Minipigsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6523-6
dc.identifier.doihttp://dx.doi.org/10.25646/6555
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirusesnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/1999-4915/12/1/38none
local.edoc.container-publisher-nameMolecular Diversity Preservation International (MDPI)none
local.edoc.container-volume12none
local.edoc.container-issue1none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage19none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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