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2019-12-04Zeitschriftenartikel DOI: 10.25646/6598
The dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinant
dc.contributor.authorBogdanow, Boris
dc.contributor.authorWang, Xi
dc.contributor.authorEichelbaum, Katrin
dc.contributor.authorSadewasser, Anne
dc.contributor.authorHusic, Immanuel
dc.contributor.authorPaki, Katharina
dc.contributor.authorBudt, Matthias
dc.contributor.authorHergeselle, Martha
dc.contributor.authorVetter, Barbara
dc.contributor.authorHou, Jingyi
dc.contributor.authorChen, Wei
dc.contributor.authorWiebusch, Lüder
dc.contributor.authorMeyer, Irmtraud M.
dc.contributor.authorWolff, Thorsten
dc.contributor.authorSelbach, Matthias
dc.date.accessioned2020-03-25T10:44:50Z
dc.date.available2020-03-25T10:44:50Z
dc.date.issued2019-12-04none
dc.identifier.other10.1038/s41467-019-13520-8
dc.identifier.urihttp://edoc.rki.de/176904/6560
dc.description.abstractPandemic influenza A virus (IAV) outbreaks occur when strains from animal reservoirs acquire the ability to infect and spread among humans. The molecular basis of this species barrier is incompletely understood. Here we combine metabolic pulse labeling and quantitative proteomics to monitor protein synthesis upon infection of human cells with a human- and a bird-adapted IAV strain and observe striking differences in viral protein synthesis. Most importantly, the matrix protein M1 is inefficiently produced by the bird-adapted strain. We show that impaired production of M1 from bird-adapted strains is caused by increased splicing of the M segment RNA to alternative isoforms. Strain-specific M segment splicing is controlled by the 3′ splice site and functionally important for permissive infection. In silico and biochemical evidence shows that avian-adapted M segments have evolved different conserved RNA structure features than human-adapted sequences. Thus, we identify M segment RNA splicing as a viral host range determinant.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectInfluenza viruseng
dc.subjectRNA splicingeng
dc.subjectSystems analysiseng
dc.subjectVirus–host interactionseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleThe dynamic proteome of influenza A virus infection identifies M segment splicing as a host range determinantnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6560-1
dc.identifier.doihttp://dx.doi.org/10.25646/6598
dc.type.versionpublishedVersionnone
local.edoc.container-titlenature communicationsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/articles/s41467-019-13520-8#article-infonone
local.edoc.container-publisher-nameSpringer Naturenone
local.edoc.container-volume10none
local.edoc.container-issue5518none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage15none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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