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2008-03-10Zeitschriftenartikel DOI: 10.1111/j.1399-3089.2008.00442.x
Knockdown of porcine endogenous retrovirus (PERV) expression by PERV-specific shRNA in transgenic pigs
dc.contributor.authorDieckhoff, Britta
dc.contributor.authorPetersen, Björn
dc.contributor.authorKues, Wilfried A.
dc.contributor.authorKurth, Reinhard
dc.contributor.authorNiemann, Heiner
dc.contributor.authorDenner, Joachim
dc.date.accessioned2018-05-07T13:55:51Z
dc.date.available2018-05-07T13:55:51Z
dc.date.created2010-06-09
dc.date.issued2008-03-10none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/recEwIhdL4ORg/PDF/29B8jplo66C.pdf
dc.identifier.urihttp://edoc.rki.de/176904/657
dc.description.abstractBackground: Xenotransplantation using porcine cells, tissues or organs may be associated with the transmission of porcine endogenous retroviruses (PERVs). More than 50 viral copies have been identified in the pig genome and three different subtypes of PERV were released from pig cells, two of them were able to infect human cells in vitro. RNA interference is a promising option to inhibit PERV transmission. Methods: We recently selected an efficient si (small interfering) RNA corresponding to a highly conserved region in the PERV DNA, which is able to inhibit expression of all PERV subtypes in PERV-infected human cells as well as in primary pig cells. Pig fibroblasts were transfected using a lentiviral vector expressing a corresponding sh (short hairpin) RNA and transgenic pigs were produced by somatic nuclear transfer cloning. Integration of the vector was proven by PCR, expression of shRNA and PERV was studied by in-solution hybridization analysis and real-time RT PCR, respectively. Results: All seven born piglets had integrated the transgene. Expression of the shRNA was found in all tissues investigated and PERV expression was significantly inhibited when compared with wild-type control animals. Conclusion: This strategy may lead to animals compatible with PERV safe xenotransplantation.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectAnimalseng
dc.subjectCellseng
dc.subjectCulturedeng
dc.subjectRNAeng
dc.subjectSwineeng
dc.subjectGenetically Modifiedeng
dc.subjectFibroblasts/cytologyeng
dc.subjectFibroblasts/physiologyeng
dc.subjectSmall Interfering/geneticseng
dc.subjectSmall Interfering/metabolismeng
dc.subjectRetroviridae*/geneticseng
dc.subjectRetroviridae*/metabolismeng
dc.subjectTransplantationeng
dc.subjectHeterologouseng
dc.subject.ddc610 Medizin
dc.titleKnockdown of porcine endogenous retrovirus (PERV) expression by PERV-specific shRNA in transgenic pigs
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-1009100
dc.identifier.doi10.1111/j.1399-3089.2008.00442.x
dc.identifier.doihttp://dx.doi.org/10.25646/582
local.edoc.container-titleXenotransplantation
local.edoc.container-textDieckhoff, B., Petersen, B., Kues, W.A., Kurth, R., Niemann, H., Denner, J. Knockdown of porcine endogenous retrovirus (PERV) expression by PERV-specific shRNA in transgenic pigs (2008) Xenotransplantation, 15 (1), pp. 36-45.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www3.interscience.wiley.com
local.edoc.container-publisher-nameWiley
local.edoc.container-volume15
local.edoc.container-issue1
local.edoc.container-year2008

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