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2020-01-29Zeitschriftenartikel DOI: 10.25646/6673
Mutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotype
dc.contributor.authorKirubakar, Greana
dc.contributor.authorSchäfer, Hubert
dc.contributor.authorRickerts, Volker
dc.contributor.authorSchwarz, Carsten
dc.contributor.authorLewin, Astrid
dc.date.accessioned2020-04-08T07:30:45Z
dc.date.available2020-04-08T07:30:45Z
dc.date.issued2020-01-29none
dc.identifier.other10.1080/21505594.2020.1713690
dc.identifier.urihttp://edoc.rki.de/176904/6623
dc.description.abstractThe lysX gene from Mycobacterium avium hominissuis (MAH) is not only involved in cationic antimicrobial resistance but also regulates metabolic activity. An MAH lysX deficient mutant was shown to exhibit a metabolic shift at the extracellular state preadapting the bacteria to the conditions inside host-cells. It further showed stronger growth in human monocytes. In the present study, the LysX activity on host–pathogen interactions were analyzed. The lysX mutant from MAH proved to be more sensitive toward host-mediated stresses such as reactive oxygen species. Further, the lysX mutant exhibited increased inflammatory response in PBMC and multinucleated giant cell (MGC) formation in human macrophages during infection studies. Coincidentally, the lysX mutant strain revealed to be more reproductive in the Galleria mellonella infection model. Together, these data demonstrate that LysX plays a role in regulating the bacillary load in host organisms and the lack of lysX gene facilitates MAH adaptation to intracellular host-habitat, thereby suggesting an essential role of LysX in the modulation of host–pathogen interaction.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectVirulenceeng
dc.subjectMycobacterium avium hominissuiseng
dc.subjectnontuberculous mycobacteriaeng
dc.subjectGalleria mellonella infection modeleng
dc.subjectmultinucleated giant cellseng
dc.subjectpathogenesiseng
dc.subjectlysX (lysyl-tRNA synthetase)eng
dc.subjecthost-pathogen interactioneng
dc.subjectinflammatory cytokineseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleMutation on lysX from Mycobacterium avium hominissuis impacts the host–pathogen interaction and virulence phenotypenone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6623-1
dc.identifier.doihttp://dx.doi.org/10.25646/6673
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirulencenone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.tandfonline.com/doi/full/10.1080/21505594.2020.1713690?scroll=top&needAccess=truenone
local.edoc.container-publisher-nameTaylor & Francisnone
local.edoc.container-volume11none
local.edoc.container-issue1none
local.edoc.container-year2020none
local.edoc.container-firstpage132none
local.edoc.container-lastpage144none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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