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2019-11-07Zeitschriftenartikel DOI: 10.25646/6718
Detection and Characterization of Human Enteroviruses, Human Cosaviruses, and a New Human Parechovirus Type in Healthy Individuals in Osun State, Nigeria, 2016/2017
dc.contributor.authorAbiodun Osundare, Folakemi
dc.contributor.authorOluyinka Opaleye, Oladele
dc.contributor.authorAbiodun Akindele, Akeem
dc.contributor.authorAdeyinka Adedokun, Samuel
dc.contributor.authorAnuoluwapo Akanbi, Olusola
dc.contributor.authorBock, Claus-Thomas
dc.contributor.authorDiedrich, Sabine
dc.contributor.authorBöttcher, Sindy
dc.date.accessioned2020-04-14T09:44:04Z
dc.date.available2020-04-14T09:44:04Z
dc.date.issued2019-11-07none
dc.identifier.other10.3390/v11111037
dc.identifier.urihttp://edoc.rki.de/176904/6666
dc.description.abstractHuman enteroviruses and human parechoviruses are associated with a broad range of diseases and even severe and fatal conditions. For human cosaviruses, the etiological role is yet unknown. Little is known about the circulation of non-polio enteroviruses, human parechoviruses, and human cosaviruses in Nigeria. A total of 113 stool samples were collected from healthy individuals in Osun State between February 2016 and May 2017. RT-PCR assays targeting the 5′ non-coding region (5′ -NCR) were used to screen for human enteroviruses, human parechoviruses, and human cosaviruses. For human enteroviruses, species-specific RT-PCR assays targeting the VP1 regions were used for molecular typing. Inoculation was carried out on RD-A, CaCo-2, HEp-2C, and L20B cell lines to compare molecular and virological assays. Ten samples tested positive for enterovirus RNA with 11 strains detected, including CV-A13 (n = 3), E-18 (n = 2), CV-A20 (n = 1), CV-A24 (n = 1), EV-C99 (n = 1), and EV-C116 (n = 2). Three samples tested positive for human parechovirus RNA, and full genome sequencing on two samples allowed assignment to a new Parechovirus A type (HPeV-19). Thirty-three samples tested positive for cosavirus with assignment to species Cosavirus D and Cosavirus A based on the 5′-NCR region. Screening of stool samples collected from healthy individuals in Nigeria in 2016 and 2017 revealed a high diversity of circulating human enteroviruses, human parechoviruses, and human cosaviruses. Molecular assays for genotyping showed substantial benefits compared with those of cell-culture assays.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectenteroviruseseng
dc.subjectparechoviruseseng
dc.subjectcosaviruseseng
dc.subjectpolioviruseng
dc.subjectcontainmenteng
dc.subjectNigeriaeng
dc.subjectpicornaviruseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleDetection and Characterization of Human Enteroviruses, Human Cosaviruses, and a New Human Parechovirus Type in Healthy Individuals in Osun State, Nigeria, 2016/2017none
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6666-1
dc.identifier.doihttp://dx.doi.org/10.25646/6718
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirusesnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/1999-4915/11/11/1037none
local.edoc.container-publisher-nameMolecular Diversity Preservation International (MDPI)none
local.edoc.container-volume11none
local.edoc.container-issue1037none
local.edoc.container-year2019none
local.edoc.container-firstpage1none
local.edoc.container-lastpage12none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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