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2020-01-21Zeitschriftenartikel DOI: 10.25646/6908
Utility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoir
dc.contributor.authorBokelmann, Marcel
dc.contributor.authorEdenborough, Kathryn
dc.contributor.authorHetzelt, Nicole
dc.contributor.authorKreher, Petra
dc.contributor.authorLander, Angelika
dc.contributor.authorNitsche, Andreas
dc.contributor.authorVogel, Uwe
dc.contributor.authorFeldmann, Heinz
dc.contributor.authorCouacy-Hymann, Emmanuel
dc.contributor.authorKurth, Andreas
dc.date.accessioned2020-05-26T12:26:21Z
dc.date.available2020-05-26T12:26:21Z
dc.date.issued2020-01-21none
dc.identifier.other10.1371/journal.pntd.0007952
dc.identifier.urihttp://edoc.rki.de/176904/6796
dc.description.abstractThe significance of the integral membrane protein Niemann-Pick C1 (NPC1) in the ebolavirus entry process has been determined using various cell lines derived from humans, non-human primates and fruit bats. Fruit bats have long been purported as the potential reservoir host for ebolaviruses, however several studies provide evidence that Mops condylurus, an insectivorous microbat, is also an ebolavirus reservoir. NPC1 receptor expression in the context of ebolavirus replication in microbat cells remains unstudied. In order to study Ebola virus (EBOV) cellular entry and replication in M. condylurus, we derived primary and immortalized cell cultures from 12 different organs. The NPC1 receptor expression was characterized by confocal microscopy and flow cytometry comparing the expression levels of M. condylurus primary and immortalized cells, HeLa cells, human embryonic kidney cells and cells from a European microbat species. EBOV replication kinetics was studied for four representative cell cultures using qRT-PCR. The aim was to elucidate the suitability of primary and immortalized cells from different tissues for studying NPC1 receptor expression levels and their potential influence on EBOV replication. The NPC1 receptor expression level in M. condylurus primary cells differed depending on the organ they were derived from and was for most cell types significantly lower than in human cell lines. Immortalized cells showed for most cell types higher expression levels than their corresponding primary cells. Concluding from our infection experiments with EBOV we suggest a potential correlation between NPC1 receptor expression level and virus replication rate in vitro.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectCell cultureseng
dc.subjectCell immortalizationeng
dc.subjectBatseng
dc.subjectFruit batseng
dc.subjectViral replicationeng
dc.subjectKidneyseng
dc.subjectPrimary cell cultureeng
dc.subjectPrimary cellseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleUtility of primary cells to examine NPC1 receptor expression in Mops condylurus, a potential Ebola virus reservoirnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6796-7
dc.identifier.doihttp://dx.doi.org/10.25646/6908
dc.type.versionpublishedVersionnone
local.edoc.container-titlePLOS Neglected Tropical Diseasesnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0007952none
local.edoc.container-publisher-namePLoSnone
local.edoc.container-volume14none
local.edoc.container-issue1none
local.edoc.container-year2020none
local.edoc.container-firstpage1none
local.edoc.container-lastpage20none
local.edoc.rki-departmentZentrum für Biologische Gefahren und Spezielle Pathogenenone
dc.description.versionPeer Reviewednone

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