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2020-03-27Zeitschriftenartikel DOI: 10.25646/6931
Search for polyoma-, herpes-, and bornaviruses in squirrels of the family Sciuridae
dc.contributor.authorSchulze, Vanessa
dc.contributor.authorLurz, Peter W. W.
dc.contributor.authorFerrari, Nicola
dc.contributor.authorRomeo, Claudia
dc.contributor.authorSteele, Michael A.
dc.contributor.authorMarino, Shealyn
dc.contributor.authorMazzamuto, Maria Vittoria
dc.contributor.authorCalvignac-Spencer, Sébastien
dc.contributor.authorSchlottau, Kore
dc.contributor.authorBeer, Martin
dc.contributor.authorUlrich, Rainer G.
dc.contributor.authorEhlers, Bernhard
dc.date.accessioned2020-05-28T13:17:54Z
dc.date.available2020-05-28T13:17:54Z
dc.date.issued2020-03-27none
dc.identifier.other10.1186/s12985-020-01310-4
dc.identifier.urihttp://edoc.rki.de/176904/6817
dc.description.abstractBackground Squirrels (family Sciuridae) are globally distributed members of the order Rodentia with wildlife occurrence in indigenous and non-indigenous regions (as invasive species) and frequent presence in zoological gardens and other holdings. Multiple species introductions, strong inter-species competition as well as the recent discovery of a novel zoonotic bornavirus resulted in increased research interest on squirrel pathogens. Therefore we aimed to test a variety of squirrel species for representatives of three virus families. Methods Several species of the squirrel subfamilies Sciurinae, Callosciurinae and Xerinae were tested for the presence of polyomaviruses (PyVs; family Polyomaviridae) and herpesviruses (HVs; family Herpesviridae), using generic nested polymerase chain reaction (PCR) with specificity for the PyV VP1 gene and the HV DNA polymerase (DPOL) gene, respectively. Selected animals were tested for the presence of bornaviruses (family Bornaviridae), using both a broad-range orthobornavirus- and a variegated squirrel bornavirus 1 (VSBV-1)-specific reverse transcription-quantitative PCR (RT-qPCR). Results In addition to previously detected bornavirus RNA-positive squirrels no more animals tested positive in this study, but four novel PyVs, four novel betaherpesviruses (BHVs) and six novel gammaherpesviruses (GHVs) were identified. For three PyVs, complete genomes could be amplified with long-distance PCR (LD-PCR). Splice sites of the PyV genomes were predicted in silico for large T antigen, small T antigen, and VP2 coding sequences, and experimentally confirmed in Vero and NIH/3T3 cells. Attempts to extend the HV DPOL sequences in upstream direction resulted in contiguous sequences of around 3.3 kilobase pairs for one BHV and two GHVs. Phylogenetic analysis allocated the novel squirrel PyVs to the genera Alpha- and Betapolyomavirus, the BHVs to the genus Muromegalovirus, and the GHVs to the genera Rhadinovirus and Macavirus. Conclusions This is the first report on molecular identification and sequence characterization of PyVs and HVs and the detection of bornavirus coinfections with PyVs or HVs in two squirrel species. Multiple detection of PyVs and HVs in certain squirrel species exclusively indicate their potential host association to a single squirrel species. The novel PyVs and HVs might serve for a better understanding of virus evolution in invading host species in the future.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectBornaviruseng
dc.subjectPolyomaviruseng
dc.subjectBetaherpesviruseng
dc.subjectGammaherpesviruseng
dc.subjectLarge Teng
dc.subjectSmall Teng
dc.subjectVP2eng
dc.subjectSplicingeng
dc.subjectSquirreleng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleSearch for polyoma-, herpes-, and bornaviruses in squirrels of the family Sciuridaenone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/6817-9
dc.identifier.doihttp://dx.doi.org/10.25646/6931
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirology Journalnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://virologyj.biomedcentral.com/articles/10.1186/s12985-020-01310-4none
local.edoc.container-publisher-nameBMCnone
local.edoc.container-volume17none
local.edoc.container-issue42none
local.edoc.container-year2020none
local.edoc.container-firstpage1none
local.edoc.container-lastpage21none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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