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2009-10-28Zeitschriftenartikel DOI: 10.1099/vir.0.016337-0
Fast broad-range disinfection of bacteria, fungi, viruses and prions
dc.contributor.authorBeekes, Michael
dc.contributor.authorLemmer, Karin
dc.contributor.authorThomzig, Achim
dc.contributor.authorJoncic, Marion
dc.contributor.authorTintelnot, Kathrin
dc.contributor.authorMielke, Martin
dc.date.accessioned2018-05-07T14:09:38Z
dc.date.available2018-05-07T14:09:38Z
dc.date.created2010-11-11
dc.date.issued2009-10-28none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reSPAPA2KTXH/PDF/25a2tE17dzW4Y.pdf
dc.identifier.urihttp://edoc.rki.de/176904/731
dc.description.abstractEffective disinfectants are of key importance for the safe handling and reprocessing of surgical instruments. We tested whether new formulations containing SDS, NaOH and 1-propanol (n-propanol) are simultaneously active against a broad range of pathogens including bacteria, fungi, non-enveloped viruses and prions. Inactivation and disinfection were examined in suspension and on carriers, respectively, using coagulated blood or brain homogenate as organic soil. Coomassie blue staining was used to assess whether formulations did undesirably fix proteins to rough surfaces. A mixture of 0.2% SDS and 0.3% NaOH in 20% n-propanol achieved potent decontamination of steel carriers contaminated with PrPTSE, the biochemical marker for prion infectivity, from 263K scrapie hamsters, or patients with sporadic or variant Creutzfeldt-Jakob disease. 263K scrapie infectivity on carriers was decreased by ≥5.5 log10 units [logs]. Furthermore, the formulation effectively inactivated poliovirus, hepatitis A virus and caliciviruses (including murine norovirus) in suspension tests. It also yielded significant titre reductions of bacteria (E. faecium, M. avium; >6 logs), fungi (spores of Aspergillus niger; >5 logs) or poliovirus (≥4 logs) embedded in coagulated blood on carriers. The formulation was not found to fix proteins more than was observed with water as cleaning reagent. SDS, NaOH and n-propanol can synergistically achieve fast broad-range disinfection.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectAnimalseng
dc.subjectHumanseng
dc.subjectBacteria/drug effectseng
dc.subjectDisinfectants/metabolismeng
dc.subjectDisinfectants/pharmacologyeng
dc.subjectDisinfection/methodseng
dc.subjectFungi/drug effectseng
dc.subjectMicrobial Viability/drug effectseng
dc.subjectPrions/drug effectseng
dc.subjectViruses/drug effectseng
dc.subject.ddc610 Medizin
dc.titleFast broad-range disinfection of bacteria, fungi, viruses and prions
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10011277
dc.identifier.doi10.1099/vir.0.016337-0
dc.identifier.doihttp://dx.doi.org/10.25646/656
local.edoc.container-titleJournal of General Virology
local.edoc.container-textThis is an author manuscript that has been accepted for publication in Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of ‘Fair Use of Copyrighted Materials’ (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://mic.sgmjournals.org, and is freely available without a subscription 12 months after publication.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://vir.sgmjournals.org/cgi/content/abstract/vir.0.016337-0v1
local.edoc.container-publisher-nameSociety for General Microbiology
local.edoc.container-volume91
local.edoc.container-year2009

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