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2009-06-15Zeitschriftenartikel DOI: 10.1099/jmm.0.011452-0
Characterization of methicillin-resistant Staphylococcus aureus (MRSA) isolates from hospitals in KwaZulu-Natal (KZN) province, Republic of South Africa
dc.contributor.authorShittu, Adebayo
dc.contributor.authorNübel, Ulrich
dc.contributor.authorUdo, Edet
dc.contributor.authorLin, Johnson
dc.contributor.authorSteven, Sedio Gaogakwe
dc.date.accessioned2018-05-07T14:11:00Z
dc.date.available2018-05-07T14:11:00Z
dc.date.created2010-11-15
dc.date.issued2009-06-15none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re1L3eLuOhG/PDF/209LwTUB9MdMs.pdf
dc.identifier.urihttp://edoc.rki.de/176904/738
dc.description.abstractEpidemiological data based on phenotypic and molecular characterization of methicillin-resistant Staphylococcus aureus (MRSA) in sub-Saharan Africa are limited. This investigation studied 61 MRSA isolates obtained from 13 health care institutions in KwaZulu-Natal province (KZN), South Africa, from March 2001 to August 2003. More than 80% of the isolates were resistant to at least four classes of antibiotics and six isolates were resistant to the aminoglycoside, macrolide-lincosamide and tetracycline groups of antibiotics, heavy metals and nucleic acid binding compounds. Pulsed field gel electrophoresis (PFGE) of SmaI digested genomic DNA revealed seven types, designated A to G. Type A was the main pulsotype (62.3%) and identified in 11 of the 13 health care institutions, suggesting that it represented a major clone in health care institutions in KZN province, South Africa. Analysis of representative members of the three major pulsotypes by spa, multilocus sequence typing (MLST) and SCCmec typing revealed the types t064-ST1173-SCCmec IV and t064-ST1338-SCCmec IV (PFGE type A, single-locus and double-locus variants of ST8), t037-ST239-SCCmec III (PFGE type F) and t045-ST5-SCCmec III (PFGE type G). The combination of various typing methods provided useful information on the geographic dissemination of MRSA clones in health care institutions in KZN, South Africa. The observation of major clones circulating in health care facilities in KZN indicates that adequate infection control measures are urgently needed.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectAnti-Bacterial Agents/pharmacologyeng
dc.subjectHumanseng
dc.subjectCross Infection/microbiologyeng
dc.subjectCross Infection/epidemiologyeng
dc.subjectBacterial Typing Techniqueseng
dc.subjectMethicillin-Resistant Staphylococcus aureus/isolation & purificationeng
dc.subjectStaphylococcal Infections/epidemiologyeng
dc.subjectSouth Africa/epidemiologyeng
dc.subjectStaphylococcal Infections/microbiology*eng
dc.subject.ddc610 Medizin
dc.titleCharacterization of methicillin-resistant Staphylococcus aureus (MRSA) isolates from hospitals in KwaZulu-Natal (KZN) province, Republic of South Africa
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10011353
dc.identifier.doi10.1099/jmm.0.011452-0
dc.identifier.doihttp://dx.doi.org/10.25646/663
local.edoc.container-titleJournal of Medical Microbiology
local.edoc.container-textThis is an author manuscript that has been accepted for publication in Microbiology, copyright Society for General Microbiology, but has not been copy-edited, formatted or proofed. Cite this article as appearing in Microbiology. This version of the manuscript may not be duplicated or reproduced, other than for personal use or within the rule of ‘Fair Use of Copyrighted Materials’ (section 17, Title 17, US Code), without permission from the copyright owner, Society for General Microbiology. The Society for General Microbiology disclaims any responsibility or liability for errors or omissions in this version of the manuscript or in any version derived from it by any other parties. The final copy-edited, published article, which is the version of record, can be found at http://mic.sgmjournals.org, and is freely available without a subscription 12 months after publication.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://jmm.sgmjournals.org/cgi/content/abstract/jmm.0.011452-0v1
local.edoc.container-publisher-nameSociety for General Microbiology
local.edoc.container-volume58
local.edoc.container-issue9
local.edoc.container-year2009

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