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2009-08-21Zeitschriftenartikel DOI: 10.1039/B911525K
Simultaneous quantification of five bacterial and plant toxins from complex matrices using a multiplexed fluorescent magnetic suspension assay
dc.contributor.authorPauly, Diana
dc.contributor.authorKirchner, Sebastian
dc.contributor.authorStoermann, Britta
dc.contributor.authorSchreiber, Tanja
dc.contributor.authorKaulfuss, Stefan
dc.contributor.authorSchade, Rüdiger
dc.contributor.authorZbinden, Reto
dc.contributor.authorAvondet, Marc-André
dc.contributor.authorDorner, Martin
dc.contributor.authorDorner, Brigitte
dc.date.accessioned2018-05-07T14:13:04Z
dc.date.available2018-05-07T14:13:04Z
dc.date.created2010-11-18
dc.date.issued2009-08-21none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reougop1eB18g/PDF/29quCMOVlIuvQ.pdf
dc.identifier.urihttp://edoc.rki.de/176904/749
dc.description.abstractProteotoxins such as ricin, abrin, botulinum neurotoxins type A and B (BoNT/A, BoNT/B) and staphylococcal enterotoxin B (SEB) are regarded as potential biological warfare agents which could be used for bioterrorism attacks on the food chain. In this study we used a novel immunisation strategy to generate high-affinity monoclonal and polyclonal antibodies against native ricin, BoNT/A, and BoNT/B. The antibodies were used along with antibodies against SEB and abrin to establish a highly sensitive magnetic and fluorescent multiplex bead array with excellent sensitivities between 2 ng/L and 546 ng/L from a minimal sample volume of 50 µL. The assay was validated using 20 different related analytes and the assay precision was determined. Advancing the existing bead array technology, the novel magnetic and fluorescent microbeads proved amenable to enrichment procedures, by further increasing sensitivity to 0.3–85 ng/L, starting from a sample volume of 500 µL. Furthermore, the method was successfully applied for the simultaneous identification of the target toxins spiked into complex food matrices like milk, baby food and yoghurt. On the basis of our results, the assay appears to be a good tool for large-scale screening of samples from the food supply chain.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectAnimalseng
dc.subjectMiceeng
dc.subjectAntibodieseng
dc.subjectTime Factorseng
dc.subjectEnzyme-Linked Immunosorbent Assayeng
dc.subjectReproducibility of Resultseng
dc.subjectInbred BALB Ceng
dc.subjectBacterial Toxins/analysiseng
dc.subjectFluorescenceeng
dc.subjectMonoclonal/immunologyeng
dc.subjectAntibody Specificityeng
dc.subjectBacterial Toxins/immunologyeng
dc.subjectFood Analysiseng
dc.subjectImmunizationeng
dc.subjectLimit of Detectioneng
dc.subjectMagneticseng
dc.subjectPlants/chemistryeng
dc.subjectSpectrometryeng
dc.subjectSuspensionseng
dc.subjectToxinseng
dc.subjectBiological/analysiseng
dc.subjectBiological/immunologyeng
dc.subject.ddc610 Medizin
dc.titleSimultaneous quantification of five bacterial and plant toxins from complex matrices using a multiplexed fluorescent magnetic suspension assay
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10011487
dc.identifier.doi10.1039/B911525K
dc.identifier.doihttp://dx.doi.org/10.25646/674
local.edoc.container-titleThe Analyst
local.edoc.container-textPauly, D., Kirchner, S., Stoermann, B., Schreiber, T., Kaulfuss, S., Schade, R., Zbinden, R., Avondet, M.-A., Dorner, M.B., Dorner, B.G. Simultaneous quantification of five bacterial and plant toxins from complex matrices using a multiplexed fluorescent magnetic suspension assay (2009) Analyst, 134 (10), pp. 2028-2039.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://pubs.rsc.org/en/Content/ArticleLanding/2009/AN/b911525k
local.edoc.container-publisher-nameRoyal Society of Chemistry
local.edoc.container-volume134
local.edoc.container-issue10
local.edoc.container-year2009

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