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2010-11-05Zeitschriftenartikel DOI: 10.1186/1743-422X-7-303
A novel adenovirus of Western lowland gorillas (Gorilla gorilla gorilla)
dc.contributor.authorWevers, Diana
dc.contributor.authorLeendertz, Fabian
dc.contributor.authorScuda, Nelly
dc.contributor.authorBoesch, Christophe
dc.contributor.authorRobbins, Martha
dc.contributor.authorHead, Josephine
dc.contributor.authorLudwig, Carsten
dc.contributor.authorKühn, Joachim
dc.contributor.authorEhlers, Bernhard
dc.date.accessioned2018-05-07T14:13:48Z
dc.date.available2018-05-07T14:13:48Z
dc.date.created2010-11-23
dc.date.issued2010-11-05none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/rerjSr6nvcgiI/PDF/28fyswzJ05fgg.pdf
dc.identifier.urihttp://edoc.rki.de/176904/753
dc.description.abstractAdenoviruses (AdV) broadly infect vertebrate hosts including a variety of primates. We identified a novel AdV in the feces of captive gorillas by isolation in cell culture, electron microscopy and PCR. From the supernatants of infected cultures we amplified DNA polymerase (DPOL), preterminal protein (pTP) and hexon gene sequences with generic pan primate AdV PCR assays. The sequences in-between were amplified by long-distance PCRs of 2 - 10 kb length, resulting in a final sequence of 15.6 kb. Phylogenetic analysis placed the novel gorilla AdV into a cluster of primate AdVs belonging to the species Human adenovirus B (HAdV-B). Depending on the analyzed gene, its position within the cluster was variable. To further elucidate its origin, feces samples of wild gorillas were analyzed. AdV hexon sequences were detected which are indicative for three distinct and novel gorilla HAdV-B viruses, among them a virus nearly identical to the novel AdV isolated from captive gorillas. This shows that the discovered virus is a member of a group of HAdV-B viruses that naturally infect gorillas. The mixed phylogenetic clusters of gorilla, chimpanzee, bonobo and human AdVs within the HAdV-B species indicate that host switches may have been a component of the evolution of human and non-human primate HAdV-B viruses.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectDNAeng
dc.subjectMolecular Sequence Dataeng
dc.subjectPhylogenyeng
dc.subjectPolymerase Chain Reactioneng
dc.subjectCluster Analysiseng
dc.subjectAnimalseng
dc.subjectMicroscopyeng
dc.subjectViral/geneticseng
dc.subjectPrimate Diseases/virologyeng
dc.subjectElectroneng
dc.subjectFeces/virologyeng
dc.subjectAdenoviridae/isolation & purificationeng
dc.subjectAdenoviridae Infections/veterinaryeng
dc.subjectGorilla gorillaeng
dc.subjectSequence Analysis DNAeng
dc.subjectAdenoviridae/classificationeng
dc.subjectAdenoviridae/geneticseng
dc.subjectAdenoviridae/ultrastructureeng
dc.subjectAdenoviridae Infections/virologyeng
dc.subjectDNA Viral/chemistryeng
dc.subjectVirion/ultrastructureeng
dc.subjectVirus Cultivationeng
dc.subject.ddc610 Medizin
dc.titleA novel adenovirus of Western lowland gorillas (Gorilla gorilla gorilla)
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10011535
dc.identifier.doi10.1186/1743-422X-7-303
dc.identifier.doihttp://dx.doi.org/10.25646/678
local.edoc.container-titleVirology Journal
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.virologyj.com/content/7/1/303
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume7
local.edoc.container-issue303
local.edoc.container-year2010

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