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2010-11-16Zeitschriftenartikel DOI: 10.1371/journal.pntd.0000883
Evaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination.
dc.contributor.authorLitzba, Nadine
dc.contributor.authorKlade, Christoph S.
dc.contributor.authorLederer, Sabine
dc.contributor.authorNiedrig, Matthias
dc.date.accessioned2018-05-07T14:17:25Z
dc.date.available2018-05-07T14:17:25Z
dc.date.created2010-12-09
dc.date.issued2010-11-16none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/regxilIFAKJmk/PDF/29YuJl899DT8Q.pdf
dc.identifier.urihttp://edoc.rki.de/176904/772
dc.description.abstractA new commercial anti-Japanese encephalitis virus IgM and IgG indirect immunofluorescence test (IIFT) was evaluated for the detection of the humoral immune response after Japanese encephalitis vaccination. The IgM IIFT was compared to two IgM capture ELISAs and the IgG IIFT was analysed in comparison to a plaque reduction neutralization test (PRNT50) and an IgG ELISA. Moreover, the course of the immune reaction after vaccination with an inactivated JEV vaccine was examined. For the present study 300 serum samples from different blood withdrawals from 100 persons vaccinated against Japanese encephalitis were used. For the IgM evaluation, altogether 78 PRNT50 positive samples taken 7 to 56 days after vaccination and 78 PRNT50 negative sera were analyzed with the Euroimmun anti-JEV IgM IIFT, the Panbio Japanese Encephalitis – Dengue IgM Combo ELISA and the InBios JE Detect IgM capture ELISA. For the IgG evaluation, 100 sera taken 56 days after vaccination and 100 corresponding sera taken before vaccination were tested in the PRNT50, the Euroimmun anti-JEV IgG IIFT, and the InBios JE Detect IgG ELISA. The Euroimmun IgM IIFT showed in comparison to the Panbio ELISA a specificity of 95% and a sensitivity of 86%. With respect to the InBios ELISA, the values were 100% and 83.9%, respectively. The analysis of the Euroimmun IgG IIFT performance and the PRNT50 results demonstrated a specificity of 100% and a sensitivity of 93.8%, whereas it was not possible to detect more than 6.6% of the PRNT50 positive sera as positive with the InBios JE Detect IgG ELISA. Thus, the IIFT is a valuable alternative to the established methods in detecting anti-JEV antibodies after vaccination in travellers and it might prove useful for the diagnosis of acutely infected persons.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectHumanseng
dc.subjectFemaleeng
dc.subjectMaleeng
dc.subjectAntibodieseng
dc.subjectImmunoglobulin M/bloodeng
dc.subjectVaccinationeng
dc.subjectEncephalitiseng
dc.subjectImmunoglobulin G/bloodeng
dc.subjectImmunoglobulin G/immunologyeng
dc.subjectImmunoglobulin M/immunologyeng
dc.subjectAntibodies Viral/bloodeng
dc.subjectViral/diagnostic useeng
dc.subjectAntibodies Viral/immunologyeng
dc.subjectEncephalitis Virus Japanese/immunologyeng
dc.subjectEncephalitis Viruseng
dc.subjectJapanese/isolation & purificationeng
dc.subjectJapanese/diagnosiseng
dc.subjectJapanese/immunologyeng
dc.subjectJapanese/prevention & controleng
dc.subjectJapanese/virologyeng
dc.subjectFluorescent Antibody Technique Indirect/methodseng
dc.subjectImmunity Humoraleng
dc.subjectImmunoglobulin G/diagnostic useeng
dc.subjectImmunoglobulin M/diagnostic useeng
dc.subjectJapanese Encephalitis Vaccines/administration & dosageeng
dc.subjectJapanese Encephalitis Vaccines/immunologyeng
dc.subject.ddc610 Medizin
dc.titleEvaluation of serological diagnostic test systems assessing the immune response to Japanese encephalitis vaccination.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10011829
dc.identifier.doi10.1371/journal.pntd.0000883
dc.identifier.doihttp://dx.doi.org/10.25646/697
local.edoc.container-titlePLoS Neglected Tropical Diseases
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosntds.org/article/info%3Adoi%2F10.1371%2Fjournal.pntd.0000883
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume4
local.edoc.container-issue11
local.edoc.container-year2010

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