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2020-11-27Zeitschriftenartikel DOI: 10.3390/jof6040319
Detection of Histoplasma DNA from Tissue Blocks by a Specific and a Broad-Range Real-Time PCR: Tools to Elucidate the Epidemiology of Histoplasmosis
dc.contributor.authorWilmes, Dunja
dc.contributor.authorMcCormick-Smith, Ilka
dc.contributor.authorLempp, Charlotte
dc.contributor.authorMayer, Ursula
dc.contributor.authorSchulze, Arik Bernard
dc.contributor.authorTheegarten, Dirk
dc.contributor.authorHartmann, Sylvia
dc.contributor.authorRickerts, Volker
dc.date.accessioned2021-02-18T07:45:20Z
dc.date.available2021-02-18T07:45:20Z
dc.date.issued2020-11-27none
dc.identifier.other10.3390/jof6040319
dc.identifier.urihttp://edoc.rki.de/176904/7804
dc.description.abstractLack of sensitive diagnostic tests impairs the understanding of the epidemiology of histoplasmosis, a disease whose burden is estimated to be largely underrated. Broad-range PCRs have been applied to identify fungal agents from pathology blocks, but sensitivity is variable. In this study, we compared the results of a specific Histoplasma qPCR (H. qPCR) with the results of a broad-range qPCR (28S qPCR) on formalin-fixed, paraffin-embedded (FFPE) tissue specimens from patients with proven fungal infections (n = 67), histologically suggestive of histoplasmosis (n = 36) and other mycoses (n = 31). The clinical sensitivity for histoplasmosis of the H. qPCR and the 28S qPCR was 94% and 48.5%, respectively. Samples suggestive for other fungal infections were negative with the H. qPCR. The 28S qPCR did not amplify DNA of Histoplasma in FFPE in these samples, but could amplify DNA of Emergomyces (n = 1) and Paracoccidioides (n = 2) in three samples suggestive for histoplasmosis but negative in the H. qPCR. In conclusion, amplification of Histoplasma DNA from FFPE samples is more sensitive with the H. qPCR than with the 28S qPCR. However, the 28S qPCR identified DNA of other fungi in H. qPCR-negative samples presenting like histoplasmosis, suggesting that the combination of both assays may improve the diagnosis.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectHistoplasma qPCReng
dc.subjectbroad-range qPCReng
dc.subjectformalin-fixed paraffin-embedded (FFPE) sampleseng
dc.subjecthistoplasmosiseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleDetection of Histoplasma DNA from Tissue Blocks by a Specific and a Broad-Range Real-Time PCR: Tools to Elucidate the Epidemiology of Histoplasmosisnone
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/7804-2
dc.identifier.doihttps://doi.org/10.3390/jof6040319
dc.identifier.doihttp://dx.doi.org/10.25646/8035
dc.type.versionpublishedVersionnone
local.edoc.container-titleJournal of Funginone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/2309-608X/6/4/319none
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume6none
local.edoc.container-issue4none
local.edoc.container-reportyear2020none
local.edoc.container-firstpage1none
local.edoc.container-lastpage12none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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