Show simple item record

2010-01-21Zeitschriftenartikel DOI: 10.1099/jmm.0.016188-0
A novel IS26 structure is surrounding blaCTX-M genes in different plasmids of German clinical isolates of Escherichia coli
dc.contributor.authorCullik, Angela
dc.contributor.authorPfeifer, Yvonne
dc.contributor.authorPrager, Rita
dc.contributor.authorBaum, Heike von
dc.contributor.authorWitte, Wolfgang
dc.date.accessioned2018-05-07T14:21:19Z
dc.date.available2018-05-07T14:21:19Z
dc.date.created2011-01-21
dc.date.issued2010-01-21none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reVCWKxH0RMF6/PDF/29ohfkz4Uujc.pdf
dc.identifier.urihttp://edoc.rki.de/176904/793
dc.description.abstractThis report focuses on the molecular characterization of 22 extended-spectrum beta-lactamase (ESBL) producing E. coli isolates collected in a German university hospital, during a period of nine months in 2006. Relationship analysis of clinical isolates was done via pulsed-field gel-electrophoresis, mulit-locus sequence typing, plasmid profiling and additionally PCR for blaESBL detection and phylogroups. After conjugal transfer plasmid isolation and subsequent PCR for blaESBL detection and incompatibility groups were performed. Using one-primer-walking, up to 3600 bp upstream and downstream of different blaCTX-M genes could be sequenced. Beta-Lactamases found were TEM-1 (n=14), SHV-5 (n=1) and a wide variety of CTX-M types (n=21) as CTX-M-15 (n=12), CTX-M-1 (n=4), CTX-M-14 (n=2), CTX-M-9 (n=1), CTX-M-3 (n=1) as well as one new type CTX-M-65 (n=1). In 18 isolates blaESBL genes were located on conjugative plasmids in sizes between 40 and 180 kbp belonging to incompatibility groups FII (n=9), N (n=5) andI1 (n=4). Thereby blaCTX-M were found to be associated with the commonly known elements ISEcp1, IS26 and IS903-D, but with unusual spacer sequences for ISEcp1 in two isolates. These insertion sequences connected to blaCTX-M as well as other genes were located between two IS26 elements in a composition that have not been described yet. The results reveal the emergence of blaESBL, preferentially blaCTX-M, located on different plasmids harboured by genotypic different E. coli strains. The identical gene arrangement in blaCTX-M neighbourhood in plasmids of different incompatibility groups indicates a main role of IS26 in distribution of mobile resistance elements between different plasmids.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectAdolescenteng
dc.subjectGeneticeng
dc.subjectHumanseng
dc.subjectEscherichia coli/geneticseng
dc.subjectEscherichia coli Proteins/geneticseng
dc.subjectFemaleeng
dc.subjectGenotypeeng
dc.subjectGermanyeng
dc.subjectElectrophoresiseng
dc.subjectGeleng
dc.subjectMaleeng
dc.subjectMiddle Agedeng
dc.subjectChildeng
dc.subjectAdulteng
dc.subjectAgedeng
dc.subjectInfanteng
dc.subjectPulsed-Fieldeng
dc.subjectYoung Adulteng
dc.subjectBacterial Typing Techniqueseng
dc.subjectConjugationeng
dc.subjectDNA Fingerprintingeng
dc.subjectChild Preschooleng
dc.subjectPlasmidseng
dc.subjectbeta-Lactamases/geneticseng
dc.subjectAged 80 and overeng
dc.subjectInfant Newborneng
dc.subjectDNA Transposable Elementseng
dc.subjectEscherichia coli/isolation & purificationeng
dc.subjectEscherichia coli Infections/microbiologyeng
dc.subjectHospitals Universityeng
dc.subjectSequence Analysis DNAeng
dc.subject.ddc610 Medizin
dc.titleA novel IS26 structure is surrounding blaCTX-M genes in different plasmids of German clinical isolates of Escherichia coli
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10012193
dc.identifier.doi10.1099/jmm.0.016188-0
dc.identifier.doihttp://dx.doi.org/10.25646/718
local.edoc.container-titleJournal of Medical Microbiology
local.edoc.container-textCullik, A., Pfeifer, Y., Prager, R., Von Baum, H., Witte, W. A novel IS26 structure surrounds blaCTX-M genes in different plasmids from German clinical Escherichia coli isolates (2010) Journal of Medical Microbiology, 59 (5), pp. 580-587.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://jmm.sgmjournals.org/cgi/content/abstract/jmm.0.016188-0v1
local.edoc.container-publisher-nameSociety for General Microbiology
local.edoc.container-volume59
local.edoc.container-issue5
local.edoc.container-year2010

Show simple item record