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2011-01-14Zeitschriftenartikel DOI: 10.1371/journal.pone.0015763
Rapid and Highly Efficient Method for Scarless Mutagenesis within the Salmonella enterica Chromosome
dc.contributor.authorBlank, Kathrin
dc.contributor.authorHensel, Michael
dc.contributor.authorGerlach, Roman
dc.date.accessioned2018-05-07T14:22:34Z
dc.date.available2018-05-07T14:22:34Z
dc.date.created2011-01-28
dc.date.issued2011-01-14none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reepc4hgEtnWI/PDF/26AlYC465KRCE.pdf
dc.identifier.urihttp://edoc.rki.de/176904/800
dc.description.abstractDirect manipulation of bacterial chromosomes by recombination-based techniques has become increasingly important for both cognitive and applied research. Here we demonstrate, for the first time, the combination of the Red recombinase system with I-SceI endonuclease-based selection of successful recombinants after electroporation with short synthetic olignucleotides. We show the generation of scarless gene knockouts as well as site-directed mutagenesis using the Salmonella virulence-associated two component signaling system PhoPQ. The presented approach is very versatile for generating in-frame deletions, point mutations or insertions within bacterial chromosomes.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectSequence Deletioneng
dc.subjectSalmonella enterica/geneticseng
dc.subjectPoint Mutationeng
dc.subjectMutagenesis Insertionaleng
dc.subjectChromosomes Bacterial/geneticseng
dc.subjectMethodseng
dc.subjectMutagenesis Site-Directed/methodseng
dc.subjectRecombination Geneticeng
dc.subject.ddc610 Medizin
dc.titleRapid and Highly Efficient Method for Scarless Mutagenesis within the Salmonella enterica Chromosome
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10012336
dc.identifier.doi10.1371/journal.pone.0015763
dc.identifier.doihttp://dx.doi.org/10.25646/725
local.edoc.container-titlePLoS One
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0015763
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume6
local.edoc.container-issue1
local.edoc.container-year2011

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