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2010-11-01Zeitschriftenartikel DOI: 10.25646/753
Virological diagnosis of dengue fever in Jeddah, Saudi Arabia: Comparison between RT-PCR and virus isolation in cell culture
dc.contributor.authorAzhar, Esam
dc.contributor.authorKao, Moujahed
dc.contributor.authorNiedrig, Matthias
dc.contributor.authorMasri, Badr
dc.contributor.authorGodus, Azad
dc.contributor.authorBadierah, Raed
dc.contributor.authorKhan, Nassem
dc.contributor.authorAlmazrooa, Adnan
dc.contributor.authorAshshi, Ahmad
dc.contributor.authorJamjoom, Ghazi
dc.date.accessioned2018-05-07T14:27:44Z
dc.date.available2018-05-07T14:27:44Z
dc.date.created2011-03-03
dc.date.issued2010-11-01none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reAVSSlt6agW2/PDF/29Abhm6vlBNY.pdf
dc.identifier.urihttp://edoc.rki.de/176904/828
dc.description.abstractA total of 233 serum samples were collected from patients presenting to King Abdulaziz University Hospital with suspected cases of Dengue Fever (DF) from 2006 to 2008. Dengue virus was successfully isolated from 70 samples by culture on C6/36 and LLC-MK2 cells; it was then detected by indirect immunofluorescence assay (IFA). The cytopathic effect (CPE) of dengue virus on C6/36 appeared in most of the samples within 1-4 days post-inoculation comparing to 7-12 days on LLC-MK2 cells, and this was characterized by the ability to induce syncytia and multinucleated giant cells. On the other hand, by using RT-PCR technique, 87 (37.3%) samples were positive. All 70 (30.4%) samples with positive cell culture results were detectable by RT-PCR in addition to 17 culture-negative samples were RT-PCR positive. Dengue virus type 1 (DENV-1) was the dominant serotype followed by DENV- 3 and DENV-2, while DENV-4 was not detected in tested samples. These results indicate that DENV-RNA detection by RT-PCR is more sensitive than virus isolation. We suggest that the high sensitivity coupled with the turnaround time, have made the RT-PCR a better choice as a routine test for DENV diagnosis.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectDengue viruseng
dc.subjectDengue fevereng
dc.subjectRT-PCReng
dc.subjectIIFeng
dc.subjectSaudi Arabiaeng
dc.subjectJeddaheng
dc.subjectDengue diagnosiseng
dc.subjectvirus isolationeng
dc.subjectvirus replicationeng
dc.subjectC6/36eng
dc.subjectLLCMK-2eng
dc.subject.ddc610 Medizin
dc.titleVirological diagnosis of dengue fever in Jeddah, Saudi Arabia: Comparison between RT-PCR and virus isolation in cell culture
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10012743
dc.identifier.doihttp://dx.doi.org/10.25646/753
local.edoc.container-titleJournal of Infectious Diseases and Immunity
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.academicjournals.org/JIDI/abstracts/abstracts/Abstracts2010/Nov/Azhar%20et%20al.htm
local.edoc.container-publisher-nameAcademic Journals
local.edoc.container-volume2
local.edoc.container-issue2
local.edoc.container-year2010

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