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2020-12-09Zeitschriftenartikel DOI: 10.25646/8880
Variable Sensitivity of SARS-CoV-2 Molecular Detection in European Expert Laboratories: External Quality Assessment, June and July 2020
dc.contributor.authorFischer, Carlo
dc.contributor.authorMögling, Ramona
dc.contributor.authorMelidou, Angeliki
dc.contributor.authorKühne, Arne
dc.contributor.authorOliveira-Filho, Edmilson F.
dc.contributor.authorWolff, Thorsten
dc.contributor.authorReiche, Janine
dc.contributor.authorBroberg, Eeva
dc.contributor.authorDrosten, Christian
dc.contributor.authorMeijer, Adam
dc.contributor.authorLeitmeyer, Katrin
dc.contributor.authorDrexler, Jan Felix
dc.contributor.authorReusken, Chantal B.E.M.
dc.date.accessioned2021-08-04T09:13:23Z
dc.date.available2021-08-04T09:13:23Z
dc.date.issued2020-12-09none
dc.identifier.otherdoi.org/10.1128/JCM.02676-20
dc.identifier.urihttp://edoc.rki.de/176904/8591
dc.description.abstractDuring the ongoing coronavirus disease 2019 (COVID-19) outbreak, robust detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a key element for clinical management and to interrupt transmission chains. We organized an external quality assessment (EQA) of molecular detection of SARS-CoV-2 for European expert laboratories. An EQA panel composed of 12 samples, containing either SARS-CoV-2 at different concentrations to evaluate sensitivity or other respiratory viruses to evaluate specificity of SARS-CoV-2 testing, was distributed to 68 laboratories in 35 countries. Specificity samples included seasonal human coronaviruses hCoV-229E, hCoV-NL63, and hCoV-OC43, as well as Middle East respiratory syndrome coronavirus (MERS-CoV), SARS-CoV, and human influenza viruses A and B. Sensitivity results differed among laboratories, particularly for low-concentration SARS-CoV-2 samples. Results indicated that performance was mostly independent of the selection of specific extraction or PCR methods.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectSARS-CoV-2eng
dc.subjectCOVID-19eng
dc.subjectEQAeng
dc.subjectmoleculareng
dc.subjectdiagnosticseng
dc.subjectEuropeeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleVariable Sensitivity of SARS-CoV-2 Molecular Detection in European Expert Laboratories: External Quality Assessment, June and July 2020none
dc.typearticle
dc.identifier.urnurn:nbn:de:kobv:0257-176904/8591-8
dc.identifier.doihttp://dx.doi.org/10.25646/8880
dc.type.versionpublishedVersionnone
local.edoc.container-titleJournal of Clinical Microbiologynone
local.edoc.container-issn1098-660Xnone
local.edoc.pages8none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://journals.asm.org/doi/10.1128/JCM.02676-20none
local.edoc.container-publisher-nameAmerican Society for Microbiologynone
local.edoc.container-volume59none
local.edoc.container-issue3none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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