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2011-05-05Zeitschriftenartikel DOI: 10.1186/1471-2180-11-92
Antibiotic resistance and molecular epidemiology of Staphylococcus aureus in Nigeria
dc.contributor.authorShittu, Adebayo
dc.contributor.authorOkon, Kenneth
dc.contributor.authorAdesida, Solayide
dc.contributor.authorOyedara, Omotayo
dc.contributor.authorWitte, Wolfgang
dc.contributor.authorStrommenger, Birgit
dc.contributor.authorLayer, Franziska
dc.contributor.authorNübel, Ulrich
dc.date.accessioned2018-05-07T14:38:29Z
dc.date.available2018-05-07T14:38:29Z
dc.date.created2011-06-14
dc.date.issued2011-05-05none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/repqS6vkHaTjE/PDF/29Q1cMqLhLgA.pdf
dc.identifier.urihttp://edoc.rki.de/176904/886
dc.description.abstractBackground: Staphylococcus aureus is an important pathogen causing a wide range of infections in the hospital and community setting. In order to have adequate information for treatment of S. aureus infections, it is crucial to understand the trends in the antibiotic-resistance patterns. In addition, the occurrence and changes in types of S. aureus, clonal identities, and their geographic spread is essential for the establishment of adequate infection control programmes. In this study, 68 S. aureus isolates obtained from clinical and non-clinical sources in Nigeria between January and April 2009 were characterized using phenotypic and molecular methods. Results: All the S. aureus isolates were susceptible to teicoplanin, vancomycin, phosphomycin, fusidic acid, rifampicin, daptomycin, mupirocin, linezolid and tigecycline. Sixteen percent of the isolates were resistant to oxacillin, while 55% and 72% of isolates were resistant to tetracycline and trimethoprim/sulphamethoxazole (cotrimoxazole), respectively (Table 1). There was excellent correlation between the broth microdilution assay and detection of antibiotic resistance genes by the multiplex PCR, in the determination of S. aureus resistance to erythromycin, gentamicin, methicillin and tetracycline. A total of 28 spa types were identified in the study, and the predominant spa type among the methicillin-susceptible S. aureus (MSSA) isolates was t084 (13 isolates). The t037- ST241-SCCmecIII type was the only clone identified in Maiduguri (North-East Nigeria) while in South-West Nigeria, diversity among the MRSA isolates (t451-ST8-SCCmecV; t008-ST94-SCCmecIV; t002-ST5-SCCmecV; t064-ST8-SCCmecV) was observed. The toxin genes seh and etd were detected in isolates affiliated with clonal complexes CC1, CC80 and sequence type ST25, respectively. The proportion of PVL-positive isolates among MSSA was high (40%). Most of the PVL-positive MSSA isolates were obtained from wound infections and associated with clonal complexes CC1, CC30, CC121 and with sequence type ST152. Conclusions: The use of phenotypic and molecular methods provided useful information on antibiotic resistance and molecular diversity of S. aureus in Nigeria. The high proportion of PVL-positive MSSA isolates affiliated to various clonal complexes and detected in all the health institutions is a major concern, both as a source of severe infections and as a potential reservoir that could lead to the emergence of PVL-positive MRSA. This study presents the first baseline information on the nature of the antibiotic resistance genes from S. aureus isolates in Nigeria. There is the need to curtail the spread and establishment of MRSA and PVL-positive MSSA clones in Nigerian health care institutions.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectAdolescenteng
dc.subjectHumanseng
dc.subjectFemaleeng
dc.subjectPolymerase Chain Reactioneng
dc.subjectCluster Analysiseng
dc.subjectMaleeng
dc.subjectMiddle Agedeng
dc.subjectNigeria/epidemiologyeng
dc.subjectChildeng
dc.subjectAdulteng
dc.subjectAgedeng
dc.subjectInfanteng
dc.subjectLeukocidins/geneticseng
dc.subjectStaphylococcus aureus/drug effectseng
dc.subjectStaphylococcus aureus/geneticseng
dc.subjectStaphylococcus aureus/isolation & purificationeng
dc.subjectYoung Adulteng
dc.subjectAnti-Bacterial Agents/pharmacologyeng
dc.subjectMolecular Epidemiologyeng
dc.subjectChild Preschooleng
dc.subjectBacterial Toxins/geneticseng
dc.subjectStaphylococcal Infections/epidemiologyeng
dc.subjectStaphylococcal Infections/microbiologyeng
dc.subjectExotoxins/geneticseng
dc.subjectInfant Newborneng
dc.subjectStaphylococcus aureus/classificationeng
dc.subjectVirulence Factors/geneticseng
dc.subjectDrug Resistance Bacterialeng
dc.subjectMolecular Typingeng
dc.subjectPhylogeographyeng
dc.subject.ddc610 Medizin
dc.titleAntibiotic resistance and molecular epidemiology of Staphylococcus aureus in Nigeria
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10014417
dc.identifier.doi10.1186/1471-2180-11-92
dc.identifier.doihttp://dx.doi.org/10.25646/811
local.edoc.container-titleBMC Microbiology
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.biomedcentral.com/1471-2180/11/92
local.edoc.container-publisher-nameBioMedCentral
local.edoc.container-volume11
local.edoc.container-issue92
local.edoc.container-year2011

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