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2011-07-14Zeitschriftenartikel DOI: 10.1371/journal.pone.0021950
Genomic expression libraries for the identification of cross-reactive orthopoxvirus antigens.
dc.contributor.authorMiller, Lilija
dc.contributor.authorRichter, Marco
dc.contributor.authorHapke, Christoph
dc.contributor.authorStern, Daniel
dc.contributor.authorNitsche, Andreas
dc.date.accessioned2018-05-07T14:42:12Z
dc.date.available2018-05-07T14:42:12Z
dc.date.created2011-08-09
dc.date.issued2011-07-14none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reAgihevzKV6/PDF/21LfRpMwLUKfM.pdf
dc.identifier.urihttp://edoc.rki.de/176904/906
dc.description.abstractIncreasing numbers of human cowpox virus infections that are being observed and that particularly affect young nonvaccinated persons have renewed interest in this zoonotic disease. Usually causing a self-limiting local infection, human cowpox can in fact be fatal for immunocompromised individuals. Conventional smallpox vaccination presumably protects an individual from infections with other Orthopoxviruses, including cowpox virus. However, available live vaccines are causing severe adverse reactions especially in individuals with impaired immunity. Because of a decrease in protective immunity against Orthopoxviruses and a coincident increase in the proportion of immunodeficient individuals in today’s population, safer vaccines need to be developed. Recombinant subunit vaccines containing cross-reactive antigens are promising candidates, which avoid the application of infectious virus. However, subunit vaccines should contain carefully selected antigens to confer a solid cross-protection against different Orthopoxvirus species. Little is known about the crossreactivity of antibodies elicited to cowpox virus proteins. Here, we first identified 21 immunogenic proteins of cowpox and vaccinia virus by serological screenings of genomic Orthopoxvirus expression libraries. Screenings were performed using sera from vaccinated humans and animals as well as clinical sera from patients and animals with a naturally acquired cowpox virus infection. We further analyzed the cross-reactivity of the identified immunogenic proteins. Out of 21 identified proteins 16 were found to be cross-reactive between cowpox and vaccinia virus. The presented findings provide important indications for the design of new-generation recombinant subunit vaccines.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Biologische Sicherheit
dc.subjectHumanseng
dc.subjectPolymerase Chain Reactioneng
dc.subjectVaccinia virus/geneticseng
dc.subjectVaccinia virus/immunologyeng
dc.subjectCowpox virus/geneticseng
dc.subjectCowpox virus/immunologyeng
dc.subjectGenome Viral/geneticseng
dc.subjectOrthopoxvirus/geneticseng
dc.subjectOrthopoxvirus/immunologyeng
dc.subjectViral Vaccines/immunologyeng
dc.subject.ddc610 Medizin
dc.titleGenomic expression libraries for the identification of cross-reactive orthopoxvirus antigens.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10014843
dc.identifier.doi10.1371/journal.pone.0021950
dc.identifier.doihttp://dx.doi.org/10.25646/831
local.edoc.container-titlePLoS ONE
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0021950
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume6
local.edoc.container-issue7
local.edoc.container-year2011

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