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2021-10-27Zeitschriftenartikel DOI: 10.25646/9497
Establishment of a Publicly Available Core Genome Multilocus Sequence Typing Scheme for Clostridium perfringens
dc.contributor.authorAbdel-Glil, Mostafa Y.
dc.contributor.authorThomas, Prasad
dc.contributor.authorLinde, Jörg
dc.contributor.authorJolley, Keith A.
dc.contributor.authorHarmsen, Dag
dc.contributor.authorWieler, Lothar H.
dc.contributor.authorNeubauer, Heinrich
dc.contributor.authorSeyboldt, Christian
dc.date.accessioned2022-01-04T08:06:00Z
dc.date.available2022-01-04T08:06:00Z
dc.date.issued2021-10-27none
dc.identifier.other10.1128/Spectrum.00533-21
dc.identifier.urihttp://edoc.rki.de/176904/9206
dc.description.abstractClostridium perfringens is a spore-forming anaerobic pathogen responsible for a variety of histotoxic and intestinal infections in humans and animals. High-resolution genotyping aiming to identify bacteria at strain level has become increasingly important in modern microbiology to understand pathogen transmission pathways and to tackle infection sources. This study aimed at establishing a publicly available genome-wide multilocus sequence-typing (MLST) scheme for C. perfringens. A total of 1,431 highly conserved core genes (1.34 megabases; 50% of the reference genome genes) were indexed for a core genome-based MLST (cgMLST) scheme for C. perfringens. The scheme was applied to 282 ecologically and geographically diverse genomes, showing that the genotyping results of cgMLST were highly congruent with the core genome-based single-nucleotide-polymorphism typing in terms of resolution and tree topology. In addition, the cgMLST provided a greater discrimination than classical MLST methods for C. perfringens. The usability of the scheme for outbreak analysis was confirmed by reinvestigating published outbreaks of C. perfringens-associated infections in the United States and the United Kingdom. In summary, a publicly available scheme and an allele nomenclature database for genomic typing of C. perfringens have been established and can be used for broad-based and standardized epidemiological studies. IMPORTANCE: Global epidemiological surveillance of bacterial pathogens is enhanced by the availability of standard tools and sharing of typing data. The use of whole-genome sequencing has opened the possibility for high-resolution characterization of bacterial strains down to the clonal and subclonal levels. Core genome multilocus sequence typing is a robust system that uses highly conserved core genes for deep genotyping. The method has been successfully and widely used to describe the epidemiology of various bacterial species. Nevertheless, a cgMLST typing scheme for Clostridium perfringens is currently not publicly available. In this study, we (i) developed a cgMLST typing scheme for C. perfringens, (ii) evaluated the performance of the scheme on different sets of C. perfringens genomes from different hosts and geographic regions as well as from different outbreak situations, and, finally, (iii) made this scheme publicly available supported by an allele nomenclature database for global and standard genomic typing.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectClostridium perfringenseng
dc.subjectSNPeng
dc.subjectcgMLSTeng
dc.subjectgenome typingeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleEstablishment of a Publicly Available Core Genome Multilocus Sequence Typing Scheme for Clostridium perfringensnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9206-1
dc.identifier.doihttp://dx.doi.org/10.25646/9497
dc.type.versionpublishedVersionnone
local.edoc.container-titleMicrobiology Spectrumnone
local.edoc.container-issn2165-0497none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://journals.asm.org/doi/full/10.1128/Spectrum.00533-21none
local.edoc.container-publisher-nameAmerican Society for Microbiologynone
local.edoc.container-volume9none
local.edoc.container-issue2none
local.edoc.container-year2021none
dc.description.versionPeer Reviewednone

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