Show simple item record

2021-01-19Zeitschriftenartikel DOI: 10.25646/9593
Genome-Based Analyses of Fitness Effects and Compensatory Changes Associated with Acquisition of blaCMY-, blaCTX-M-, and blaOXA-48/VIM-1-Containing Plasmids in Escherichia coli
dc.contributor.authorPietsch, Michael
dc.contributor.authorPfeifer, Yvonne
dc.contributor.authorFuchs, Stephan
dc.contributor.authorWerner, Guido
dc.date.accessioned2022-01-31T09:28:12Z
dc.date.available2022-01-31T09:28:12Z
dc.date.issued2021-01-19none
dc.identifier.other10.3390/antibiotics10010090
dc.identifier.urihttp://edoc.rki.de/176904/9288
dc.description.abstractBackground: Resistance plasmids are under selective conditions beneficial for the bacterial host, but in the absence of selective pressure, this carriage may cause fitness costs. Compensation of this fitness burden is important to obtain competitive ability under antibiotic-free conditions. In this study, we investigated fitness effects after a conjugative transfer of plasmids containing various beta-lactamase genes transferred into Escherichia coli. (2) Methods: Fourteen beta-lactamase-encoding plasmids were transferred from clinical donor strains to E. coli J53. Growth rates were compared for all transconjugants and the recipient. Selected transconjugants were challenged in long-term growth experiments. Growth rates were assessed at different time points during growth for 500 generations. Whole-genome sequencing (WGS) of initial and evolved transconjugants was determined. Results: Most plasmid acquisitions resulted in growth differences, ranging from −4.5% to 7.2%. Transfer of a single blaCMY-16-carrying plasmid resulted in a growth burden and a growth benefit in independent mating. Long-term growth led to a compensation of fitness burdens and benefits. Analyzing WGS revealed genomic changes caused by Single Nucleotide Polymorphisms (SNPs) and insertion sequences over time. Conclusions: Fitness effects associated with plasmid acquisitions were variable. Potential compensatory mutations identified in transconjugants’ genomes after 500 generations give interesting insights into aspects of plasmid–host adaptationseng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectAmpCeng
dc.subjectESBLeng
dc.subjectcarbapenemaseeng
dc.subjectfitness burdeneng
dc.subjectcompensatory mutationeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleGenome-Based Analyses of Fitness Effects and Compensatory Changes Associated with Acquisition of blaCMY-, blaCTX-M-, and blaOXA-48/VIM-1-Containing Plasmids in Escherichia colinone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9288-7
dc.identifier.doihttp://dx.doi.org/10.25646/9593
dc.type.versionpublishedVersionnone
local.edoc.container-titleantibioticsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/2079-6382/10/1/90none
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume10none
local.edoc.container-issue1none
local.edoc.container-year2021none
local.edoc.container-firstpage1none
local.edoc.container-lastpage18none
dc.description.versionPeer Reviewednone

Show simple item record