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2011-04-01Zeitschriftenartikel DOI: 10.1371/journal.pone.0018345
Presence and seeding activity of pathological prion protein (PrP(TSE)) in skeletal muscles of white-tailed deer infected with chronic wasting disease.
dc.contributor.authorDaus, Martin L.
dc.contributor.authorBreyer, Johanna
dc.contributor.authorWagenführ, Katja
dc.contributor.authorWemheuer, Wiebke M.
dc.contributor.authorThomzig, Achim
dc.contributor.authorSchulz-Schaeffer, Walter
dc.contributor.authorBeekes, Michael
dc.date.accessioned2018-05-07T14:46:15Z
dc.date.available2018-05-07T14:46:15Z
dc.date.created2011-09-02
dc.date.issued2011-04-01none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/re0Pt0kzJ2p2/PDF/24RmQcjdMYm9Q.pdf
dc.identifier.urihttp://edoc.rki.de/176904/928
dc.description.abstractChronic wasting disease (CWD) is a contagious, rapidly spreading transmissible spongiform encephalopathy (TSE), or prion disease, occurring in cervids such as white tailed-deer (WTD), mule deer or elk in North America. Despite efficient horizontal transmission of CWD among cervids natural transmission of the disease to other species has not yet been observed. Here, we report for the first time a direct biochemical demonstration of pathological prion protein PrPTSE and of PrPTSE-associated seeding activity, the static and dynamic biochemical markers for biological prion infectivity, respectively, in skeletal muscles of CWD-infected cervids, i. e. WTD for which no clinical signs of CWD had been recognized. The presence of PrPTSE was detected by Western- and postfixed frozen tissue blotting, while the seeding activity of PrPTSE was revealed by protein misfolding cyclic amplification (PMCA). Semi-quantitative Western blotting indicated that the concentration of PrPTSE in skeletal muscles of CWD-infected WTD was approximately 2000-10000 -fold lower than in brain tissue. Tissue-blot-analyses revealed that PrPTSE was located in muscle-associated nerve fascicles but not, in detectable amounts, in myocytes. The presence and seeding activity of PrPTSE in skeletal muscle from CWD-infected cervids suggests prevention of such tissue in the human diet as a precautionary measure for food safety, pending on further clarification of whether CWD may be transmissible to humans.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectAnimalseng
dc.subjectWesterneng
dc.subjectBlottingeng
dc.subjectDeer/metabolismeng
dc.subjectMuscle Skeletal/metabolismeng
dc.subjectPrions/chemistryeng
dc.subjectPrions/isolation & purificationeng
dc.subjectPrions/metabolismeng
dc.subjectProtein Multimerizationeng
dc.subjectProtein Structure Quaternaryeng
dc.subjectProtein Transporteng
dc.subjectWasting Disease Chronic/metabolismeng
dc.subject.ddc610 Medizin
dc.titlePresence and seeding activity of pathological prion protein (PrP(TSE)) in skeletal muscles of white-tailed deer infected with chronic wasting disease.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10015123
dc.identifier.doi10.1371/journal.pone.0018345
dc.identifier.doihttp://dx.doi.org/10.25646/853
local.edoc.container-titlePLoS ONE
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0018345
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume6
local.edoc.container-issue4
local.edoc.container-year2011

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