Show simple item record

2021-04-08Zeitschriftenartikel
Innovative and Highly Sensitive Detection of Clostridium perfringens Enterotoxin Based on Receptor Interaction and Monoclonal Antibodies
dc.contributor.authorNeumann, Thea
dc.contributor.authorKrüger, Maren
dc.contributor.authorWeisemann, Jasmin
dc.contributor.authorMahrhold, Stefan
dc.contributor.authorStern, Daniel
dc.contributor.authorDorner, Martin B.
dc.contributor.authorFeraudet-Tarisse, Cécile
dc.contributor.authorPöhlmann, Christopher
dc.contributor.authorSchulz, Katharina
dc.contributor.authorMesselhäußer, Ute
dc.contributor.authorRimek, Dagmar
dc.contributor.authorGessler, Frank
dc.contributor.authorElßner, Thomas
dc.contributor.authorSimon, Stéphanie
dc.contributor.authorRummel, Andreas
dc.contributor.authorDorner, Brigitte G.
dc.date.accessioned2022-02-01T08:31:55Z
dc.date.available2022-02-01T08:31:55Z
dc.date.issued2021-04-08none
dc.identifier.other10.3390/toxins13040266
dc.identifier.urihttp://edoc.rki.de/176904/9304
dc.description.abstractClostridium perfringens enterotoxin (CPE) regularly causes food poisoning and antibioticassociated diarrhea; therefore, reliable toxin detection is crucial. To this aim, we explored stationary and mobile strategies to detect CPE either exclusively by monoclonal antibodies (mAbs) or, alternatively, by toxin-enrichment via the cellular receptor of CPE, claudin-4, and mAb detection. Among the newly generated mAbs, we identified nine CPE-specific mAbs targeting five distinct epitopes, among them mAbs recognizing CPE bound to claudin-4 or neutralizing CPE activity in vitro. In surface plasmon resonance experiments, all mAbs and claudin-4 revealed excellent affinities towards CPE, ranging from 0.05 to 2.3 nM. Integrated into sandwich enzyme-linked immunosorbent assays (ELISAs), the most sensitive mAb/mAb and claudin-4/mAb combinations achieved similar detection limits of 0.3 pg/mL and 1.0 pg/mL, respectively, specifically detecting recombinant CPE from spiked feces and native CPE from 30 different C. perfringens culture supernatants. The implementation of mAb- and receptor-based ELISAs into a mobile detection platform enabled the fast detection of CPE, which will be helpful in clinical laboratories to diagnose diarrhea of assumed bacterial origin. In conclusion, we successfully employed an endogenous receptor and novel high affinity mAbs for highly sensitive and specific CPE-detection. These tools will be useful for both basic and applied research.eng
dc.language.isofranone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectC. perfringens enterotoxin CPEeng
dc.subjectClostridium perfringenseng
dc.subjectmonoclonal antibodieseng
dc.subjectreceptor claudin-4eng
dc.subjectstationary lab-based detectioneng
dc.subjectmobile on-site detectioneng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleInnovative and Highly Sensitive Detection of Clostridium perfringens Enterotoxin Based on Receptor Interaction and Monoclonal Antibodiesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9304-6
dc.type.versionpublishedVersionnone
local.edoc.container-titleToxinsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/2072-6651/13/4/266none
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume13none
local.edoc.container-issue4none
local.edoc.container-year2021none
local.edoc.container-firstpage1none
local.edoc.container-lastpage24none
local.edoc.rki-departmentZentrum für Biologische Gefahren und Spezielle Pathogenenone
dc.description.versionPeer Reviewednone

Show simple item record