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2021-06-29Zeitschriftenartikel
Detection of Q129H Immune Escape Mutation in Apparently Healthy Hepatitis B Virus Carriers in Southwestern Nigeria
dc.contributor.authorAdesina, Olufisayo Adeyemi
dc.contributor.authorAkanbi, Olusola Anuoluwapo
dc.contributor.authorOpaleye, Oluyinka Oladele
dc.contributor.authorJaphet, Margaret Oluwatoyin
dc.contributor.authorWang, Bo
dc.contributor.authorOluyege, Adekemi Olubukunola
dc.contributor.authorKlink, Patrycja
dc.contributor.authorBock, C.-Thomas
dc.date.accessioned2022-02-01T14:36:50Z
dc.date.available2022-02-01T14:36:50Z
dc.date.issued2021-06-29none
dc.identifier.other10.3390/v13071273
dc.identifier.urihttp://edoc.rki.de/176904/9322
dc.description.abstractAs the global effort to eradicate hepatitis B continues, immune escape mutations (IEMs) and drug resistance mutations (DRMs) affecting its diagnosis, treatment, and prevention are compromising this goal. However, knowledge about the prevalence and circulation of these mutations in Nigeria is scarce. Serum samples (n = 199) from apparently healthy prospective blood donors, pregnant women, and individuals presenting with fever in southwestern Nigeria were analyzed for the presence of IEMs and DRMs by means of nested PCR in the HBV S (HBs) and HBV polymerase (Pol) genes, followed by phylogenetic and mutational analyses. In total, 25.1% (n = 50/199) of samples were positive for HBV, as measured by PCR. In 41 samples (20.6%), both fragments could be amplified, whereas the HBs gene and the Pol gene fragment alone were detected in 0.5% (n = 1/199) and 4% (n = 8/199) of samples, respectively. Sequences were successfully obtained for all 42 HBs gene fragments but for only 31/49 Pol gene fragments (totaling 73 sequences from 44 individuals). All sequences were identified as HBV genotype E. IEMs were present in 18.2% (n = 8/44) of the sequences of HBV-positive individuals with available sequences. IEM Q129H was detected in eight out of the 44 (18.2%) HBV isolates sequenced in this study; however, no DRMs were observed. This study confirms the circulation of HBV IEMs and reports the presence of Q129H IEM for the first time in Nigeria. Intensified research on the dynamics of IEM is necessary in order to enhance the elimination of HBV.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjecthepatitiseng
dc.subjectmutationeng
dc.subjectresistanceeng
dc.subjectPCReng
dc.subjectphylogenyeng
dc.subjectNigeriaeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleDetection of Q129H Immune Escape Mutation in Apparently Healthy Hepatitis B Virus Carriers in Southwestern Nigerianone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9322-6
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirusesnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/1999-4915/13/7/1273none
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume13none
local.edoc.container-issue7none
local.edoc.container-year2021none
local.edoc.container-firstpage1none
local.edoc.container-lastpage14none
local.edoc.rki-departmentInfektionskrankheitennone
dc.description.versionPeer Reviewednone

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