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2021-04-01Zeitschriftenartikel
The virome ofGerman bats: comparing virus discovery approaches
dc.contributor.authorKohl, Claudia
dc.contributor.authorBrinkmann, Annika
dc.contributor.authorRadonić, Aleksandar
dc.contributor.authorDabrowski, Piotr Wojtek
dc.contributor.authorMühldorfer, Kristin
dc.contributor.authorNitsche, Andreas
dc.contributor.authorWibbelt, Gudrun
dc.contributor.authorKurth, Andreas
dc.date.accessioned2022-02-02T12:57:30Z
dc.date.available2022-02-02T12:57:30Z
dc.date.issued2021-04-01none
dc.identifier.other10.1038/s41598-021-86435-4
dc.identifier.urihttp://edoc.rki.de/176904/9346
dc.description.abstractBats are known to be reservoirs of several highly pathogenic viruses. Hence, the interest in bat virus discovery has been increasing rapidly over the last decade. So far, most studies have focused on a single type of virus detection method, either PCR, virus isolation or virome sequencing. Here we present a comprehensive approach in virus discovery, using all three discovery methods on samples from the same bats. By family-specific PCR screening we found sequences of paramyxoviruses, adenoviruses, herpesviruses and one coronavirus. By cell culture we isolated a novel bat adenovirus and bat orthoreovirus. Virome sequencing revealed viral sequences of ten different virus families and orders: three bat nairoviruses, three phenuiviruses, one orbivirus, one rotavirus, one orthoreovirus, one mononegavirus, five parvoviruses, seven picornaviruses, three retroviruses, one totivirus and two thymoviruses were discovered. Of all viruses identified by family-specific PCR in the original samples, none was found by metagenomic sequencing. Vice versa, none of the viruses found by the metagenomic virome approach was detected by family-specific PCRs targeting the same family. The discrepancy of detected viruses by different detection approaches suggests that a combined approach using different detection methods is necessary for virus discovery studies.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleThe virome ofGerman bats: comparing virus discovery approachesnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9346-4
dc.type.versionpublishedVersionnone
local.edoc.container-titleScientific Reportsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/articles/s41598-021-86435-4none
local.edoc.container-publisher-nameSpringer Naturenone
local.edoc.container-volume11none
local.edoc.container-year2021none
local.edoc.container-firstpage1none
local.edoc.container-lastpage18none
local.edoc.rki-departmentZentrum für Biologische Gefahren und Spezielle Pathogenenone
dc.description.versionPeer Reviewednone

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