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2021-10-25Zeitschriftenartikel
Third generation cephalosporin resistance in clinical nontyphoidal Salmonella enterica in Germany and emergence of blaCTX-M-harbouring pESI plasmids
dc.contributor.authorPietsch, Michael
dc.contributor.authorSimon, Sandra
dc.contributor.authorMeinen, Anika
dc.contributor.authorTrost, Eva
dc.contributor.authorBanerji, Sangeeta
dc.contributor.authorPfeifer, Yvonne
dc.contributor.authorFlieger, Antje
dc.date.accessioned2022-02-02T13:29:26Z
dc.date.available2022-02-02T13:29:26Z
dc.date.issued2021-10-25none
dc.identifier.other10.1099/mgen.0.000698
dc.identifier.urihttp://edoc.rki.de/176904/9348
dc.description.abstractNon-typhoidal Salmonella enterica is an important gastrointestinal pathogen causing a considerable burden of disease. Resistance to third generation cephalosporins poses a serious threat for treatment of severe infections. In this study occurrence, phylogenetic relationship, and mechanisms of third generation cephalosporin resistance were investigated for clinical non-typhoidal S. enterica isolates in Germany. From 2017 to 2019, we detected 168 unique clinical S. enterica isolates with phenotypic resistance to third generation cephalosporins in a nation-wide surveillance. Compared to previous years, we observed a significant (P=0.0002) and consistent increase in resistant isolates from 0.41 % in 2005 to 1.71 % in 2019. In total, 34 different serovars were identified, most often S. Infantis (n=41; 24.4 %), S. Typhimurium (n=27; 16.1 %), S. Kentucky (n=21; 12.5 %), and S. Derby (n=17; 10.1 %). Whole genome analyses revealed extended-spectrum β-lactamase (ESBL) genes as main cause for third generation cephalosporin resistance, and most prevalent were blaCTX-M-1 (n=55), blaCTX-M-14 (n=25), and blaCTX-M-65 (n=23). There was no strict correlation between serovar, phylogenetic lineage, and ESBL type but some serovar/ESBL gene combinations were detected frequently, such as blaCTX-M-1 and blaCTX-M-65 in S. Infantis or blaCTX-M-14b in S. Kentucky. The ESBL genes were mainly located on plasmids, including IncI, IncA/C variants, emerging pESI variants, and a novel blaCTX-M-1harbouring plasmid. We conclude that third generation cephalosporin resistance is on the rise among clinical S. enterica isolates in Germany, and occurrence in various S. enterica serovars is most probably due to multiple acquisition events of plasmids.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectSalmonella entericaeng
dc.subjectantibiotic resistanceeng
dc.subjectextended-spectrum β-lactamase (ESBL)eng
dc.subjectplasmideng
dc.subjectpESIeng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleThird generation cephalosporin resistance in clinical nontyphoidal Salmonella enterica in Germany and emergence of blaCTX-M-harbouring pESI plasmidsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9348-3
dc.type.versionpublishedVersionnone
local.edoc.container-titleMicrobial Genomicsnone
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.microbiologyresearch.org/content/journal/mgen/10.1099/mgen.0.000698#tab2none
local.edoc.container-publisher-nameMicrobiology Societynone
local.edoc.container-volume7none
local.edoc.container-issue10none
local.edoc.container-year2021none
local.edoc.container-firstpage1none
local.edoc.container-lastpage10none
dc.description.versionPeer Reviewednone

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