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2021-07-30Zeitschriftenartikel
Rapid and Robust Continuous Purification of High-Titer Hepatitis B Virus for In Vitro and In Vivo Applications
dc.contributor.authorWettengel, Jochen M.
dc.contributor.authorLinden, Bianca
dc.contributor.authorEsser, Knud
dc.contributor.authorLaue, Michael
dc.contributor.authorBurwitz, Benjamin J.
dc.contributor.authorProtzer, Ulrike
dc.date.accessioned2022-02-16T10:47:29Z
dc.date.available2022-02-16T10:47:29Z
dc.date.issued2021-07-30none
dc.identifier.other10.3390/v13081503
dc.identifier.urihttp://edoc.rki.de/176904/9427
dc.description.abstractAvailable treatments for hepatitis B can control the virus but are rarely curative. This led to a global initiative to design new curative therapies for the 257 million patients affected. Discovery and development of these new therapies is contingent upon functional in vitro and in vivo hepatitis B virus (HBV) infection models. However, low titer and impurity of conventional HBV stocks reduce significance of in vitro infections and moreover limit challenge doses in current in vivo models. Therefore, there is a critical need for a robust, simple and reproducible protocol to generate high-purity and high-titer infectious HBV stocks. Here, we outline a three-step protocol for continuous production of high-quality HBV stocks from supernatants of HBV-replicating cell lines. This purification process takes less than 6 h, yields to high-titer stocks (up to 1 × 1011 enveloped, DNA-containing HBV particles/mL each week), and is with minimal equipment easily adaptable to most laboratory settings.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjecthepatitis B viruseng
dc.subjectvirus purificationeng
dc.subjectheparin-affinity chromatographyeng
dc.subjectsucrose-gradient ultracentrifugationeng
dc.subjectHBV high-titer virus stockseng
dc.subjectHBV for in vitro and in vivo assayseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleRapid and Robust Continuous Purification of High-Titer Hepatitis B Virus for In Vitro and In Vivo Applicationsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9427-4
dc.type.versionpublishedVersionnone
local.edoc.container-titleVirusesnone
local.edoc.container-issn1999-4915none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/1999-4915/13/8/1503/htmnone
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume13none
local.edoc.container-issue8none
local.edoc.container-year2021none
dc.description.versionPeer Reviewednone

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