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2022-01-29Zeitschriftenartikel
Phenotypic Diversity of Cryptococcus neoformans var. neoformans Clinical Isolates from Localized and Disseminated Infections
dc.contributor.authorBirkenfeld, Zena M.
dc.contributor.authorDittel, Nikita
dc.contributor.authorHarrer, Thomas
dc.contributor.authorStephan, Christoph
dc.contributor.authorKiderlen, Albrecht F.
dc.contributor.authorRickerts, Volker
dc.date.accessioned2022-03-17T08:46:22Z
dc.date.available2022-03-17T08:46:22Z
dc.date.issued2022-01-29none
dc.identifier.other10.3390/microorganisms10020321
dc.identifier.urihttp://edoc.rki.de/176904/9513
dc.description.abstractCryptococcus neoformans var. neoformans is the second most prevalent agent of cryptococcosis in central Europe. Infections mostly present with localized skin and disseminated infections. Previous studies did not find these presentations to be determined by the fungal genotype as detected by multilocus sequence typing (MLST). However, phenotypic fungal traits may impact clinical presentation. Here, we studied the growth and virulence factors of C. neoformans var. neoformans isolates from disseminated and localized infections and an environmental isolate. We used coincubation with Acanthamoeba castellanii and the Galleria mellonella infection model to identify phenotypic characteristics potentially associated with clinical presentation. Clinical isolates of C. neoformans var. neoformans present a substantial phenotypic variability. Median survival of G. mellonella varied between 6 and 14 days. C. neoformans var. neoformans isolates from disseminated infections showed stronger melanization and larger capsules. They demonstrated superior uptake into an amoeba and increased cytotoxicity for the amoeba. Differences of strains from localized and disseminated infections in coincubation with amoeba are in line with the importance of phagocytes in the pathogenesis of disseminated cryptococcosis. Phenotypic traits and non-vertebrate infection models may help understand the virulence potential of C. neoformans var. neoformans isolates.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectcryptococcosiseng
dc.subjectCryptococcus neoformanseng
dc.subjectGalleria mellonellaeng
dc.subjectAcanthamoeba castellaniieng
dc.subjectvirulence factorseng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titlePhenotypic Diversity of Cryptococcus neoformans var. neoformans Clinical Isolates from Localized and Disseminated Infectionsnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9513-5
dc.type.versionpublishedVersionnone
local.edoc.container-titleMicroorganismsnone
local.edoc.container-issn2076-2607none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.mdpi.com/2076-2607/10/2/321none
local.edoc.container-publisher-nameMDPInone
local.edoc.container-volume10none
local.edoc.container-issue2none
local.edoc.container-year2022none
dc.description.versionPeer Reviewednone

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