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2022-03-04Zeitschriftenartikel
In vitro maturation of Toxoplasma gondii bradyzoites in human myotubes and their metabolomic characterization
dc.contributor.authorChristiansen, Céline
dc.contributor.authorMaus, Deborah
dc.contributor.authorHoppenz, Ellen
dc.contributor.authorMurillo-León, Mateo
dc.contributor.authorHoffmann, Tobias
dc.contributor.authorScholz, Jana
dc.contributor.authorMelerowicz, Florian
dc.contributor.authorSteinfeldt, Tobias
dc.contributor.authorSeeber, Frank
dc.contributor.authorBlume, Martin
dc.date.accessioned2022-04-06T06:44:21Z
dc.date.available2022-04-06T06:44:21Z
dc.date.issued2022-03-04none
dc.identifier.other10.1038/s41467-022-28730-w
dc.identifier.urihttp://edoc.rki.de/176904/9593
dc.description.abstractThe apicomplexan parasite Toxoplasma gondii forms bradyzoite-containing tissue cysts that cause chronic and drug-tolerant infections. However, current in vitro models do not allow long-term culture of these cysts to maturity. Here, we developed a human myotube-based in vitro culture model of functionally mature tissue cysts that are orally infectious to mice and tolerate exposure to a range of antibiotics and temperature stresses. Metabolomic characterization of purified cysts reveals global changes that comprise increased levels of amino acids and decreased abundance of nucleobase- and tricarboxylic acid cycle-associated metabolites. In contrast to fast replicating tachyzoite forms of T. gondii these tissue cysts tolerate exposure to the aconitase inhibitor sodium fluoroacetate. Direct access to persistent stages of T. gondii under defined cell culture conditions will be essential for the dissection of functionally important host-parasite interactions and drug evasion mechanisms. It will also facilitate the identification of new strategies for therapeutic intervention.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleIn vitro maturation of Toxoplasma gondii bradyzoites in human myotubes and their metabolomic characterizationnone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9593-0
dc.type.versionpublishedVersionnone
local.edoc.container-titlenature communicationsnone
local.edoc.container-issn2041-1723none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.nature.com/articles/s41467-022-28730-wnone
local.edoc.container-publisher-nameNature Researchnone
local.edoc.container-volume13none
local.edoc.container-issue1none
local.edoc.container-year2022none
dc.description.versionPeer Reviewednone

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