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2022-04-14Zeitschriftenartikel
N-chlorotaurine is highly active against respiratory viruses including SARS-CoV-2 (COVID-19) in vitro
dc.contributor.authorLackner, Michaela
dc.contributor.authorRössler, Annika
dc.contributor.authorVolland, André
dc.contributor.authorStadtmüller, Marlena Nastassja
dc.contributor.authorMüllauer, Brigitte
dc.contributor.authorBanki, Zoltan
dc.contributor.authorStröhle, Johannes
dc.contributor.authorLuttick, Angela
dc.contributor.authorFenner, Jennifer
dc.contributor.authorSarg, Bettina
dc.contributor.authorKremser, Leopold
dc.contributor.authorTone, Paul
dc.contributor.authorStoiber, Heribert
dc.contributor.authorvon Laer, Dorothee
dc.contributor.authorWolff, Thorsten
dc.contributor.authorSchwarz, Carsten
dc.contributor.authorNagl, Markus
dc.date.accessioned2022-05-16T06:48:28Z
dc.date.available2022-05-16T06:48:28Z
dc.date.issued2022-04-14none
dc.identifier.other10.1080/22221751.2022.2065932
dc.identifier.urihttp://edoc.rki.de/176904/9718
dc.description.abstractN-chlorotaurine (NCT) a long-lived oxidant generated by leukocytes, can be synthesized chemically and applied topically as an anti-infective to different body sites, including the lung via inhalation. Here, we demonstrate the activity of NCT against viruses causing acute respiratory tract infections, namely severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), influenza viruses, and respiratory syncytial virus (RSV). Virucidal activity of NCT was tested in plaque assays, confirmed by RT-qPCR assays. Attack on virus proteins was investigated by mass spectrometry. NCT revealed broad virucidal activity against all viruses tested at 37°C and pH 7. A significant reduction in infectious particles of SARS-CoV-2 isolates from early 2020 by 1 log10 was detected after 15 min of incubation in 1% NCT. Proteinaceous material simulating body fluids enhanced this activity by transchlorination mechanisms (1 -2 log10 reduction within 1–10 minutes). Tested SARS-CoV-2 variants B.1.1.7 (Alpha) und B.1.351 (Beta) showed a similar susceptibility. Influenza virus infectious particles were reduced by 3 log10 (H3N2) to 5 log10 (H1N1pdm), RSV by 4 log10. Mass spectrometry of NCT-treated SARS-CoV-2 spike protein and 3C-like protease, influenza virus hemagglutinin and neuraminidase, and RSV fusion glycoprotein disclosed multiple sites of chlorination and oxidation as the molecular mechanism of action. Application of 1.0% NCT as a prophylactic and therapeutic strategy against acute viral respiratory tract infections deserves comprehensive clinical investigation.eng
dc.language.isoengnone
dc.publisherRobert Koch-Institut
dc.rights(CC BY 3.0 DE) Namensnennung 3.0 Deutschlandger
dc.rights.urihttp://creativecommons.org/licenses/by/3.0/de/
dc.subjectN-chlorotaurineeng
dc.subjectCOVID-19eng
dc.subjectinfluenzaeng
dc.subjectrespiratory syncytial viruseng
dc.subjectantiviraleng
dc.subjectanti-infectiveeng
dc.subjectantisepticeng
dc.subjectrespiratory tracteng
dc.subject.ddc610 Medizin und Gesundheitnone
dc.titleN-chlorotaurine is highly active against respiratory viruses including SARS-CoV-2 (COVID-19) in vitronone
dc.typearticle
dc.identifier.urnurn:nbn:de:0257-176904/9718-7
dc.type.versionpublishedVersionnone
local.edoc.container-issn2222-1751none
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttps://www.tandfonline.com/doi/full/10.1080/22221751.2022.2065932none
local.edoc.container-publisher-nameTaylor & Francisnone
local.edoc.container-year2022none
local.edoc.container-periodicalpart-titleEmerging Microbes & Infectionsnone
local.edoc.container-firstpage1none
local.edoc.container-lastpage49none
dc.description.versionPeer Reviewednone

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