Show simple item record

2011-09-30Zeitschriftenartikel DOI: 10.1074/mcp.M111.010157
Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti - identification of stage-specific proteases.
dc.contributor.authorSoblik, Hanns
dc.contributor.authorYounis, Abuelhassan Elshazly
dc.contributor.authorMitreva, Makedonka
dc.contributor.authorRenard, Bernhard Y.
dc.contributor.authorKirchner, Marc
dc.contributor.authorGeisinger, Frank
dc.contributor.authorSteen, Hanno
dc.contributor.authorBrattig, Norbert W.
dc.date.accessioned2018-05-07T15:08:42Z
dc.date.available2018-05-07T15:08:42Z
dc.date.created2012-01-30
dc.date.issued2011-09-30none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reiqKmrYoJgrA/PDF/21tUJmhoUCpU.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1051
dc.description.abstractA wide range of biomolecules, including proteins, are excreted and secreted from helminths and contribute to the parasite's successful establishment, survival, and reproduction in an adverse habitat. Excretory and secretory proteins (ESP) are active at the interface between parasite and host and comprise potential targets for intervention. The intestinal nematode Strongyloides spp. exhibits an exceptional developmental plasticity in its life cycle characterized by parasitic and free-living generations. We investigated ESP from infective larvae, parasitic females, and free-living stages of the rat parasite Strongyloides ratti, which is genetically very similar to the human pathogen, Strongyloides stercoralis. Proteomic analysis of ESP revealed 586 proteins, with the largest number of stage-specific ESP found in infective larvae (196), followed by parasitic females (79) and free-living stages (35). One hundred and forty proteins were identified in all studied stages, including anti-oxidative enzymes, heat shock proteins, and carbohydrate-binding proteins. The stage-selective ESP of (1) infective larvae included an astacin metalloproteinase, the L3 Nie antigen, and a fatty acid retinoid-binding protein; (2) parasitic females included a prolyl oligopeptidase (prolyl serine carboxypeptidase), small heat shock proteins, and a secreted acidic protein; (3) free-living stages included a lysozyme family member, a carbohydrate-hydrolyzing enzyme, and saponin-like protein. We verified the differential expression of selected genes encoding ESP by qRT-PCR. ELISA analysis revealed the recognition of ESP by antibodies of S. ratti-infected rats. A prolyl oligopeptidase was identified as abundant parasitic female-specific ESP, and the effect of pyrrolidine-based prolyl oligopeptidase inhibitors showed concentration- and time-dependent inhibitory effects on female motility. The characterization of stage-related ESP from Strongyloides will help to further understand the interaction of this unique intestinal nematode with its host.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectAmino Acid Sequenceeng
dc.subjectGene Expression Regulationeng
dc.subjectMolecular Sequence Dataeng
dc.subjectFemaleeng
dc.subjectMaleeng
dc.subjectAnimalseng
dc.subjectBase Sequenceeng
dc.subjectRatseng
dc.subjectProtease Inhibitors/pharmacologyeng
dc.subjectProteomicseng
dc.subjectCulture Media/chemistryeng
dc.subjectDevelopmentaleng
dc.subjectHelminth Proteins/geneticseng
dc.subjectHelminth Proteins/metabolismeng
dc.subjectHelminth Proteins/secretioneng
dc.subjectImmune Sera/chemistryeng
dc.subjectIntestines/parasitologyeng
dc.subjectLarva/enzymologyeng
dc.subjectLarva/geneticseng
dc.subjectLarva/growth & developmenteng
dc.subjectPeptide Hydrolases/geneticseng
dc.subjectPeptide Hydrolases/metabolismeng
dc.subjectPeptide Hydrolases/secretioneng
dc.subjectProtein Sorting Signalseng
dc.subjectProtein Structure Tertiaryeng
dc.subjectRats Wistareng
dc.subjectReal-Time Polymerase Chain Reactioneng
dc.subjectSequence Analysis Proteineng
dc.subjectSerine Endopeptidases/geneticseng
dc.subjectSerine Endopeptidases/metabolismeng
dc.subjectSerine Endopeptidases/secretioneng
dc.subjectStatistics Nonparametriceng
dc.subjectStrongyloides ratti/enzymologyeng
dc.subjectStrongyloides ratti/geneticseng
dc.subjectStrongyloides ratti/growth & developmenteng
dc.subjectStrongyloidiasis/parasitologyeng
dc.subject.ddc610 Medizin
dc.titleLife cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti - identification of stage-specific proteases.
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10018065
dc.identifier.doi10.1074/mcp.M111.010157
dc.identifier.doihttp://dx.doi.org/10.25646/976
local.edoc.container-titleMolecular & Cellular Proteomics
local.edoc.container-textSoblik, H., Younis, A.E., Mitreva, M., Renard, B.Y., Kirchner, M., Geisinger, F., Steen, H., Brattig, N.W. Life cycle stage-resolved proteomic analysis of the excretome/secretome from Strongyloides ratti - Identification of stage-specific proteases (2011) Molecular and Cellular Proteomics, 10 (12)
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.mcponline.org/content/10/12/M111.010157
local.edoc.container-publisher-nameThe American Society for Biochemistry and Molecular Biology, Inc.
local.edoc.container-volume10
local.edoc.container-issue12
local.edoc.container-year2011

Show simple item record