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2012-06-29Zeitschriftenartikel DOI: 10.1371/journal.pone.0040004
Rapid Discrimination of Salmonella enterica Serovar Typhi from Other Serovars by MALDI-TOF Mass Spectrometry
dc.contributor.authorKuhns, Martin
dc.contributor.authorZautner, Andreas E.
dc.contributor.authorRabsch, Wolfgang
dc.contributor.authorZimmermann, Ortrud
dc.contributor.authorWeig, Michael
dc.contributor.authorBader, Oliver
dc.contributor.authorGroß, Uwe
dc.date.accessioned2018-05-07T15:47:17Z
dc.date.available2018-05-07T15:47:17Z
dc.date.created2012-08-22
dc.date.issued2012-06-29none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reTEiRjna7sIA/PDF/23udLZfmAhb.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1262
dc.description.abstractSystemic infections caused by Salmonella enterica are an ongoing public health problem especially in Sub-Saharan Africa. Essentially typhoid fever is associated with high mortality particularly because of the increasing prevalence of multidrug-resistant strains. Thus, a rapid blood-culture based bacterial species diagnosis including an immediate sub-differentiation of the various serovars is mandatory. At present, MALDI-TOF based intact cell mass spectrometry (ICMS) advances to a widely used routine identification tool for bacteria and fungi. In this study, we investigated the appropriateness of ICMS to identify pathogenic bacteria derived from Sub-Saharan Africa and tested the potential of this technology to discriminate S. enterica subsp. enterica serovar Typhi (S. Typhi) from other serovars. Among blood culture isolates obtained from a study population suffering from febrile illness in Ghana, no major misidentifications were observed for the species identification process, but serovars of Salmonella enterica could not be distinguished using the commercially available Biotyper database. However, a detailed analysis of the mass spectra revealed several serovar-specific biomarker ions, allowing the discrimination of S. Typhi from others. In conclusion, ICMS is able to identify isolates from a sub-Saharan context and may facilitate the rapid discrimination of the clinically and epidemiologically important serovar S. Typhi and other non-S. Typhi serovars in future implementations.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut, Infektionskrankheiten / Erreger
dc.subjectHumanseng
dc.subjectPhylogenyeng
dc.subjectSerotypingeng
dc.subjectBacterial Typing Techniques/methodseng
dc.subjectSpecies Specificityeng
dc.subjectSalmonella typhi/classificationeng
dc.subjectSalmonella typhi/geneticseng
dc.subjectSalmonella typhi/isolation & purificationeng
dc.subjectProteomicseng
dc.subjectSpectrometry Mass Matrix-Assisted Laser Desorption-Ionization/methodseng
dc.subject.ddc610 Medizin
dc.titleRapid Discrimination of Salmonella enterica Serovar Typhi from Other Serovars by MALDI-TOF Mass Spectrometry
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10026669
dc.identifier.doi10.1371/journal.pone.0040004
dc.identifier.doihttp://dx.doi.org/10.25646/1187
local.edoc.container-titlePLoS ONE
local.edoc.container-textKuhns M, Zautner AE, Rabsch W, Zimmermann O, Weig M, et al. (2012) Rapid Discrimination of Salmonella enterica Serovar Typhi from Other Serovars by MALDI-TOF Mass Spectrometry. PLoS ONE 7(6): e40004.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0040004
local.edoc.container-publisher-namePublic Library of Science
local.edoc.container-volume7
local.edoc.container-issue6
local.edoc.container-year2012

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