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2009-07-22Zeitschriftenartikel DOI: 10.1099/vir.0.013524-0
Measles viruses of genotype H1 evade recognition by vaccine-induced neutralizing antibodies targeting the linear haemagglutinin noose epitope
dc.contributor.authorFinsterbusch, Tim
dc.contributor.authorWolbert, Anne
dc.contributor.authorDeitemeier, Ingrid
dc.contributor.authorMeyer, Kerstin
dc.contributor.authorMosquera, Maria Mar
dc.contributor.authorMankertz, Annette
dc.contributor.authorSantibanez, Sabine
dc.date.accessioned2018-05-07T16:02:45Z
dc.date.available2018-05-07T16:02:45Z
dc.date.created2012-11-05
dc.date.issued2009-07-22none
dc.identifier.otherhttp://edoc.rki.de/oa/articles/reSjEv3TeGwj/PDF/24ecGkogWhh3A.pdf
dc.identifier.urihttp://edoc.rki.de/176904/1346
dc.description.abstractThe linear haemagglutinin noose epitope (HNE; aa 379-410) is a protective B-cell epitope and considered to be highly conserved in both the vaccine and the wild-type measles virus (MeV) haemagglutinin (H) proteins. Vaccine virus-derived monoclonal antibodies (mAbs) BH6 and BH216, which target the HNE, neutralized MeVs of genotypes B3, C2, D4, D5, D6, D7 and D8, and the vaccine strain Edmonston Zagreb. In the case of genotype H1, only strain Berlin.DEU/44.01 was neutralized by these mAbs, whereas strains Shenyang.CHN/22.99 and Sofia.BGR/19.05 were not. The H gene sequences of these two strains showed an exchange of proline 397 (P397) to leucine (L397). Mutated H proteins, with P397 exchanged to L and vice versa, were compared with original H proteins by indirect fluorescence assay. H proteins exhibiting P397 but not those with L397 were recognized by BH6 and BH216. This indicates that L397 leads to the loss of the neutralizing HNE. In contrast, human sera obtained from vaccinees (n=10) did not discriminate between genotype H1 variants P397 and L397. This concurs with the epidemiological observation that the live-attenuated vaccine protects against both H1 variants. Furthermore, we demonstrated that MeVs of genotype H1 also lack the neutralizing epitopes defined by the vaccine virus-induced mAbs BH15, BH125 and BH47. The loss of several neutralizing epitopes, as shown for H1 viruses currently circulating endemically in Asia, implies that epitope monitoring should be considered to be included in measles surveillance.eng
dc.language.isoeng
dc.publisherRobert Koch-Institut
dc.subjectAmino Acid Sequenceeng
dc.subjectHumanseng
dc.subjectMolecular Sequence Dataeng
dc.subjectGenotypeeng
dc.subjectMeasles virus/geneticseng
dc.subjectNeutralization Testseng
dc.subjectSequence Analysis DNAeng
dc.subjectMutation Missenseeng
dc.subjectRNA Viral/geneticseng
dc.subjectAsiaeng
dc.subjectAntibodies Viral/immunologyeng
dc.subjectMeasles virus/isolation & purificationeng
dc.subjectMeasles/virologyeng
dc.subjectMeasles virus/immunologyeng
dc.subjectEpitopes/immunologyeng
dc.subjectHemagglutinins Viral/geneticseng
dc.subjectHemagglutinins Viral/immunologyeng
dc.subjectMeasles Vaccine/immunologyeng
dc.subjectMutant Proteins/immunologyeng
dc.subject.ddc610 Medizin
dc.titleMeasles viruses of genotype H1 evade recognition by vaccine-induced neutralizing antibodies targeting the linear haemagglutinin noose epitope
dc.typeperiodicalPart
dc.identifier.urnurn:nbn:de:0257-10027784
dc.identifier.doi10.1099/vir.0.013524-0
dc.identifier.doihttp://dx.doi.org/10.25646/1271
local.edoc.container-titleJournal of General Virology
local.edoc.container-textTim Finsterbusch, Anne Wolbert, Ingrid Deitemeier, Kerstin Meyer, Maria Mar Mosquera, Annette Mankertz and Sabine Santibanez. Measles viruses of genotype H1 evade recognition by vaccine-induced neutralizing antibodies targeting the linear haemagglutinin noose epitope. (2009) Journal of General Virology, 90 (11), pp. 2739-2745.
local.edoc.fp-subtypeArtikel
local.edoc.type-nameZeitschriftenartikel
local.edoc.container-typeperiodical
local.edoc.container-type-nameZeitschrift
local.edoc.container-urlhttp://vir.sgmjournals.org/content/90/11/2739
local.edoc.container-publisher-nameSociety for General Microbiology
local.edoc.container-volume90
local.edoc.container-issue11
local.edoc.container-year2009

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